用PCR方法定量检测多药抗性基因的表达  

Quantitative Analysis of Multidrug Resistance Gene Expression by Polymerase Chain Reaction

作　　者：汲言山[1] 沈倍奋[1] 王文香[1] 郭静竹[2] 李锦云[2] 

机构地区：[1]军事医学科学院基础所,北京100850 [2]北京医科大学人民医院

出　　处：《生物化学杂志》1993年第3期326-330,共5页

摘　　要：肿瘤细胞对化疗药物的抗性是癌症有效化疗的主要障碍。人类细胞中多药抗性基因(MDR1)编码一种p-糖蛋白,后者功能是能量依赖的跨膜药物外输泵,可降低细胞毒药物在胞内的积累。定量分析MDR1表达水平可说明病人抗药能力的高低。本文应用PCR技术建立了灵敏度高、专一性好、可定量检测临床标本中MDR1表达水平的方法。对周血标本的初步检测表明:白血病化疗效果与MDR1表达水平的高低有关。The presence of drug resistant malignant cells is a major obstacle to effective cancer chemotherpy. One mechanism of drug resistance is associated with over expression of multidrug resistance gene (MDR1) and phenotypic expression of a trans-membrane P-glycoprotein, which acts as an efflux pump and resulting in decreased intracellular drug accumulation. In order to improve the theraputic effect for individual patients, we devised a highly sentitive, specific and quantitive protocol for measuring the levels of MDR1 mRNA in mononuclear cells in peripheral blood or bone marrow of leukemia patients, based on the PCR. Our results demonstrated that the expression of MDR1 seems to be correlated with the response to chemotherapy.

关 键 词：多药抗性基因 聚合酶链反应 抗药性 

分 类 号：R394.8[医药卫生—医学遗传学]