Cycloheximide prevents production of arresting,a fraction of 30-50 kDa obtained from seminiferous tubule conditioned medium  

作　　者：Gustavo F.Gonzales Stella Hartinger Yetssy Flores Kelly Zárate Pedro Yi 

机构地区：[1]Instituto de Investigations de la Altura,Department of Biological and Physiological Sciences,Department of Biological and Physiological Sciences,Department of Biological and Physiological Sciences,Faculty of Veterinary Medicine and Animal Sciences Department of Biological and Physiological Sciences, and, and, and, and, Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima, Peru

出　　处：《Asian Journal of Andrology》2004年第4期359-364,共6页亚洲男性学杂志（英文版）

摘　　要：Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P<0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P<0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.

关 键 词：CYCLOHEXIMIDE ARRESTING conditioned medium seminiferous tubule sperm production TRANSILLUMINATION 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]