单唾液酸神经节苷脂对大鼠脑血管痉挛后局部脑血流量与微血管的影响  

Effect of GM_1 on rCBF and microvessel after cerebral vasospasm in rats

作　　者：任传成[1] 王云甫[2] 谢连红[1] 周亚刚[1] 钱湘绮[1] 余桂军[1] 周玲[1] 倪冬艳[1] 

机构地区：[1]复旦大学附属上海市第五人民医院神经内科,200240 [2]华中科技大学同济医学院

出　　处：《临床神经病学杂志》2004年第6期435-437,共3页Journal of Clinical Neurology

基　　金：上海市卫生局科研发展基金项目 (编号 :0 2 4 0 99)

摘　　要：目的　观察单唾液酸神经节苷脂 (GM1)对脑血管痉挛 (CVS)后局部脑血流量 (rCBF)与微血管的影响 ,以探讨其防治CVS的可能性。方法　将 2 5只大鼠随机分为对照组、CVS组、尼莫地平组 (Nim组 )、GM1治疗组 (GM1 T组 )、GM1预防组 (GM1 P组 )。运用蛛网膜下腔二次注血制备CVS模型。每组分别于二次注血后 0h、0 5h、1h、3h及 6h测量rCBF ;6 5h后断头取脑 ,抗Ⅷ因子相关抗原免疫组化染色标记微血管 ,图像分析观察颞叶皮质微血管的数密度、面密度。结果　 (1)rCBF :二次注血后CVS组、Nim组、GM1 T组的rCBF明显下降 (均P <0 0 1) ,6h时Nim组、GM1 T组的rCBF回升至注血前水平 ,CVS组的rCBF无明显回升 ;GM1 P组在二次注血后rCBF无明显下降。 (2 )图像分析 :CVS组的微血管数目、面积及平均面积均减少 (均P <0 0 1) ;Nim组的微血管面积较对照组、CVS组明显增加 (均P <0 0 1) ;GM1 T组的微血管数目、面积及平均面积较对照组、CVS组显著增加 (均P <0 0 1) ;GM1 P组的微血管数目、面积及平均面积增多最明显 ,与CVS组比较差异有极显著性 (均P <0 0 1)。结论　蛛网膜下腔二次注血可降低rCBF、减少皮质微血管数目和面积 ,诱发CVS,尼莫地平可缓解CVS程度 ,但GM1的治疗作用比尼莫地平更强 。Objective To investigate the effect of GM 1 on vasospasm after subarachnoid hemorrhage.Methods Twenty-five wistar rats receiving intracisternal injection of 0.15 ml autologous nonheparinized blood or 0.15 ml saline two times were divided into five groups as follows: control group, cerebral vasospasm (CVS) group, Nimodipine group, GM 1 therapy group and GM 1 prevent group. The cortical regional cerebral blood flow (rCBF) was measured by hydrogenate clearance method at 0, 0.5, 1, 3 and 6h respectively. After perfusion and fixation, capillary vessels in temporal lobe cortex were labeled with anti-factor Ⅷ and observed by microvascular graphic analysis. Results (1) Compared with control group, rCBFs in CVS group, Nimodipine group and GM 1 therapy group were apparently decreased after second intracisternal blood injection (all P<0.01), but was not apparently decreased in GM 1 prevent group. 6h after second intracisternal blood injection, rCBFs were increased in Nimodipine group and GM 1 therapy group, but no apparent increase in CVS group. (2) Compared with control group, total microvascular areas, microvascular counts and mean microvascular areas were decreased in CVS group (all P<0.01). They were apparently increased in Nimodipine group, GM 1 therapy group and GM 1 prevent group (all P<0.01).Conclusions CVS may be caused by intracisternal blood injections two times, and GM 1 may prevent and relieve cerebral vasospasm. GM 1 is better than Nimodipine in treatment of cerebral vasospasm.

关 键 词：脑血管痉挛 局部脑血流量 神经节苷脂 尼莫地平 微血管图像分析 

分 类 号：R743[医药卫生—神经病学与精神病学]