大鼠脑出血后脑组织转铁蛋白表达的实验研究  

Experimental study on expression of transferrin in brain tissue after intracerebral hemorrhage in rats

作　　者：刘韶华[1] 姜亚军[2] 吴颢昕[3] 周红[1] 林靓[3] 陈雪琴[3] 

机构地区：[1]东南大学附属中大医院神经内科,南京210009 [2]南京中医药大学附属江苏省中医院脑病中心 [3]南京中医药大学基础医学院

出　　处：《临床神经病学杂志》2004年第6期438-440,共3页Journal of Clinical Neurology

基　　金：江苏省社会发展基金 (BS2 0 0 0 - 82 )

摘　　要：目的　探讨大鼠自发性脑出血 (ICH)后不同时间脑组织中转铁蛋白 (Tf)的表达及其规律。方法　采用立体定向技术注入自体不凝血建立实验性大鼠ICH模型 ,在不同时间断头取脑 ,免疫组化染色检测脑组织中Tf阳性细胞的表达并与对照组比较。结果　ICH后 6h血肿周围及同侧大脑皮质Tf阳性细胞的表达增高 ,72h达高峰 ,然后下降 ,但第 7d时仍高于对照组 (P <0 0 5 )。结论　ICH后血肿周围和同侧大脑皮质Tf阳性细胞表达增高 。Objective To investigate the expression and its rule of transferrin (Tf) in brain tissue at different time after experimental intracerebral hemorrhage (ICH) in rats. Methods The fresh quantitative autologous blood was infused into the right caudate nucleus of rat sterotaxically to build up experimental ICH model. At 6h, 24h, 72h and 7d after operation, the rats were sacrificed and the brain tissues were made for immunohistochemistry analysis of Tf. The water content of the brains was also assayed.Results Compared with saline group, Tf-immunostaining positive cells in the tissue surrounding hematoma and in the ipsilateral pallium in ICH rats were increased significantly during 7 days, peaked at 72 hours ( P<0.05).Conclusion Up-regulation of Tf in the brain tissue surrounding hematoma and in the ipsilateral pallium may provide protective effect on neurons and relieve delayed brain edema in ICH rats.

关 键 词：脑出血 转铁蛋白 免疫组化染色 

分 类 号：R743.34[医药卫生—神经病学与精神病学]