小鼠异位心脏移植模型的建立  

Establishing the model of mice heart heterotopic transplantation

作　　者：吴蔚[1] 杨康[1] 姜恒[1] 

机构地区：[1]第三军医大学西南医院胸心外科,重庆400038

出　　处：《重庆医学》2004年第12期1777-1778,共2页Chongqing medicine

摘　　要：目的　为方便现代移植免疫学研究 ,我们建立了小鼠异位心脏移植模型 ,为今后的器官移植研究打下基础。方法　以C5 7小鼠为受体 ,Balb/c小鼠为供体 ,参照Ono法并加以改良 ,将供体升主动脉、肺动脉与受体腹主动脉和下腔静脉行端侧连续吻合。结果　正式试验 2 5次 ,4例在 72h以内死亡 ,其余 2 1例均长期存活 ,手术成功率为 84 %。结论　在熟练掌握显微外科技术的基础上 ,处理好心脏移植的 5个关键步骤 ,小鼠异位心脏移植模型即可顺利建立。Objective To make the study of transplantation immunobiology more convenience, we establish the model of mice heart heterotopic transplantation.Methods Using the Kunming mice for experiment, we anastomosised the aorta and pulmonary artery of donor's heart with the abdominal aorta and inferior cava of the receptor respectively according to the Onok method.Results We did pre-experiments 50 times and formal experiments 50times. The survival rate was 84%. Conclusions In the base of mastering the technique of operation facility, if we handed the five procedures successfully, the model of mice heart heterotopic transplantation would be established auspicious.

关 键 词：小鼠 心脏 异位移植 

分 类 号：R-332[医药卫生]