用于羊毛细化的酸性蛋白酶产生菌的选育  

Study on the screening of strains with high activity acid protease for wool

作　　者：陈德兆[1] 张同亮[1] 张扬[1] 

机构地区：[1]东华大学化学化工学院,上海200051

出　　处：《毛纺科技》2004年第11期19-22,共4页Wool Textile Journal

摘　　要：利用紫外线和亚硝酸对黑曲霉进行联合诱变,使用自制的培养基筛选出7株对羊毛细化具有效果的菌株,又通过摇瓶复筛出1株对羊毛细化具有明显效果的菌株。同时设计了1种以羊毛作为底物的酶活力的测定方法,用这种测定方法,筛选出的菌株的最高产酶活力可以达到620μg ml。A high yield acid protease producing strain Asp.niger DH-C40 is screened from its parent strain Asp.niger DH-010 by treating with UV and nitrous acid. The acid protease is good at decompose wool. Invent a new way to determine the activity of the enzyme for wool. Define 1 ml enzyme decompose 1 μg wool in one hour as 1 unit activity of enzyme. A new screening culture medium was designed. Using the new culture medium, 7 strains which can produce the enzyme for wool are screened. Using the flask bottle to screen among the 7 strains, we get a high yield strain——DH-C40. Using the new determine way, DH-40 can produce a high yield acid protease for wool, the activity of protease is 620 μg/ml.

关 键 词：羊毛细化 菌种选育 酸性蛋白酶 酶活测定 

分 类 号：TS101.4[轻工技术与工程—纺织工程]