急性淋巴细胞白血病外周血WT1基因表达的意义  

Detection Wilm′s Tumor Gene Expression in Peripheral Blood and Its Clinical Significance of Acute Lymphocytic Leukemia Children

作　　者：李荣[1] 聂常富[1] 王建国[1] 郭志华[1] 

机构地区：[1]新乡医学院第一附属医院,河南卫辉453100

出　　处：《实用儿科临床杂志》2005年第1期27-28,共2页Journal of Applied Clinical Pediatrics

基　　金：河南省科委科技攻关项目 (981 1 70 2 33)

摘　　要：目的　建立realtimeRT PCR检测WT1基因表达方法 ,了解急性淋巴细胞白血病 (ALL)患儿外周血WT1基因表达水平。方法　建立realtimeRT PCR方法 ,采用PEABI 770 0PCR仪检测ALL 37例患儿、非白血病 1 3例和 1 8例正常儿童外周血WT1基因表达水平。结果　ALL初发 1 3例WT1基因的表达量为 (1 0 5～ 1 0 6 )拷贝 / μgRNA ,部分缓解 1 2例ALL患者的表达水平为 (1 0 2 ～ 1 0 4 )拷贝 / μgRNA ,ALL完全缓解 1 2例表达水平为 (0～ 1 0 2 )拷贝 / μgRNA。 结论　WT1基因在ALL外周血中有高水平表达 ,可作为疗效考核及监测微残留病指标。Objective To establish a real time reverse transcription polymerase chain reaction method for detecting WT1 and to understand the expression levels of WT1 in acute lymphocytic leukemia(ALL) of children through examining peripheral blood of leukemia children.Methods Thirty ALL patients, 13 non-leukemia Children and 18 normal children were included in this study. The method of real time RT-PCR detecting the expression of WT1 was established. The expression levels of WT1 gene were tested by this method.Results The expression levels of WT1 in 13 ALL with newly diagnosed patients were (105-106)copies/μg RNA, 12 with partial remission were (102-104)copies/μg RNA and 12 with complete remission were (0-102)copies/μg RNA.Conclusions Significant expression levels of WT1 in ALL are higher than those in non-leukemias and normal children.WT1 could be a marker for detecting minimal residual disease and evaluating therapy efficacy in ALL.

关 键 词：白血病 急性淋巴细胞 Wilm’s瘤基因 REAL time逆转录聚合酶链反应 

分 类 号：R733.71[医药卫生—肿瘤]