假单胞菌菌苗免疫调节和抑瘤活性的实验研究  

Effects of Pseudomonas vaccine (DLPV) treatment on immune functions and tumor growth in mice

作　　者：王冰[1] 于芒[1] 

机构地区：[1]大连医科大学组胚教研室,辽宁大连116027

出　　处：《中国微生态学杂志》2004年第6期329-332,共4页Chinese Journal of Microecology

摘　　要：应用假单胞菌菌苗(DLPV)皮下注射来调节荷瘤小鼠的免疫功能。结果发现DLPV能解除小鼠荷瘤状态所抑制的自然杀伤细胞的细胞毒活性以及脾脏单个核细胞的增殖活力;同时发现,DLPV能够显著提高荷瘤小鼠腹腔巨噬细胞的细胞毒活性。其结果,经DLPV治疗的荷瘤小鼠,其体内的移植瘤的生长受到明显抑制(肿瘤生长抑制率达到4135%)。通过测定血清和瘤体中谷胱甘肽硫转移酶(GST)和γ谷氨酰转移酶(γGT)的活性,也证实了DLPV的抑制作用。DLPV was derived from a strain of Pseudomonas aeruginosa peripherally expressing mannose-sensitive hemaglutinin pili.To test the effects of DLPV treatment on immune functions and tumor growth,male BALB/c mice were subcutaneously(s.c.)inoculated with 6×106 murine malignant hepatoma Hca-F25/CL-16A 3(16A 3) cells in the left inguen.Ten days after the inoculation,those mice were injected s.c. with either 0 5 ml normal saline (NS) or equal volume of DLPV(5×108 paraformaldehyde-treated bacterial cells in NS), once two days for 10 times.One week after the last treatment,animals were sacrificed.It was found that the growth of tumor was significantly inhibited by DLPV treatment (by 41 35% in tumor weight,P<0 05,compared with tumor-bearing controls,n=8),which was associated with significantly increased cytotoxic activities of natural killer (NK) cells and peritoneal macrophage (PM) against tumor cells, as well as greatly enhanced ConA-stimulated splenocyte proliferation.Measurement of the levels of glutathione S-transferase (GST) and gamma glutamyltransferase (GGT) in the serum and tumor tissue also confirmed the anti-tumor effect of DLPV.

关 键 词：肿瘤 假单胞菌菌苗 巨噬细胞 自然杀伤细胞 谷胱甘肽-硫转移酶 Γ-谷氨酰转移酶 免疫调节活性 抑瘤活性 生物反应调节剂 疗效 

分 类 号：R730.3[医药卫生—肿瘤]