白血病骨髓基质细胞中GATA-1和GATA-2基因的表达特点  

Expressions of Transcription Factor GATA-1 and GATA-2 Genes in Bone Marrow Stromal Cells from Patients with Leukemia

作　　者：吴秀丽[1] 李扬秋[1] 王震[1] 杨力建[1] 陈少华[1] 张洹[1] 朱康儿[2] 韩忠朝[3] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632 [2]暨南大学附属一院血液科,广州510632 [3]中国医学科学院,中国协和医科大学血液研究所,天津300020

出　　处：《中国实验血液学杂志》2005年第1期70-75,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(3970064);国家攀登计划资助项目(95专10);广东省自然科学基金(970627);国家教委留学回国人员启动基金(教外司留[1998]679号)

摘　　要：为了解转录因子GATA 1和GATA 2基因在白血病和正常骨髓基质细胞 (BMSC)中表达的情况 ,采集了4 2例白血病患者的骨髓标本及 34例正常骨髓标本并分离骨髓单个核细胞 ,在体外扩增培养后收集悬浮细胞 (骨髓细胞 )和扩增后的贴壁细胞 (BMSC) ,应用RT PCR ELISA方法分别检测GATA 1和GATA 2基因的表达情况 ,并对其相对表达水平进行半定量分析。结果发现 ,GATA 1和GATA 2基因在正常和白血病的BMSC和骨髓细胞中均有一定的表达。在BMSC中急性淋巴细胞白血病 (ALL)组的GATA 1基因表达率 (85 .7% )与正常组 (88.2 % )相近 ,而急性髓性白血病 (AML)和慢性粒细胞白血病 (CML)组的GATA 1表达率 (5 5 .6 %和 4 1.2 % )均比正常组低(P =0 .0 0 8,0 .0 0 0 ) ,且ALL的BMSC中GATA 1基因表达水平 >AML >正常 >CML(P均 <0 .0 5 ) ;而各白血病组BMSC中GATA 2的表达率和表达水平与正常组相比均无显著性差异 (P均 >0 .0 5 )。骨髓细胞中AML组的GATA 1基因表达水平 >正常 >ALL >CML ,AML组的GATA 2基因表达水平 >CML >ALL >正常 (P均 <0 .0 5 )。AML、CML和正常组的BMSC和骨髓细胞中均以GATA 2的表达占优势。可以推论 ,转录因子GATA 1和GATA 2基因在正常和白血病BMSC的表达可能影响骨髓微环境的造血调控作用。是否对白血病的发生?In order to investigate expressions of transcription factor GATA-1 and GATA-2 genes in the bone marrow stromal cells (BMSCs) from patients with leukemia or normal controls, bone marrow stromal cells from 34 normal cases and 42 cases with leukemia were cultured long-term in vitro. Nonadherent cells (bone marrow hematopoietic cells) and amplified adherent cells (BMSC) were collected separately. Expressions of GATA-1 and GATA-2 genes were analyzed by using RT-PCR-ELISA; the semi-quantitative expression levels of GATA genes in the BMSCs from patients with leukemia were compared with normal controls. The results showed that expressions of GATA-1 and GATA-2 genes could be detec ted in the BMSCs and the bone marrow hematopoietic cells from both normal controls and the cases of leukemia. The expression ratio of GATA-1 in the BMSCs from acute lymphocytic leukemia (ALL) (85.7%) was similar to the normal controls (88.2%), whereas the expression ratios in BMSCs from acute myelocytic leukemia (AML) (55.6%) and chronic myelocytic leukemia (CML) (41.2%) were significant lower than the normal controls (P<0.05). The rank of expression level of GATA-1 gene in the BMSCs was “ALL>AML>normal>CML”. There was no difference in the expression level of GATA-2 gene within the BMSCs from normal controls and patients with leukemia. The ranks of expression levels of GATA-1 and GATA-2 genes in bone marrow hematopoietic cells were “AML>normal>ALL>CML” and “AML> CML>ALL>normal”. The dominant expression of GATA-2 gene was found in the BMSCs from AML, CML or normal controls. It is inferred that the expressions of GATA-1 and GATA-2 genes in the BMSCs of normal controls and patients with leukemia may influence the regulation of hematopoiesis in the bone marrow stroma and it is worthy of further study to explore their roles in pathogenesis and development of leukemia.

关 键 词：转录因子 GATA-1 GATA-2 白血病 骨髓基质细胞 基因表达 

分 类 号：R733.7[医药卫生—肿瘤]