FISH应用于植入前遗传学诊断对高风险胚胎检测的研究  

The exploring of the application of fluorescent in-situ hybridization in preimplantation genetic diagnosis

作　　者：陆小溦[1] 冯云 张爱军[1] 孙贻娟[1] 

机构地区：[1]上海第二医科大学附属瑞金医院生殖医学中心,200025

出　　处：《中国优生与遗传杂志》2005年第1期54-56,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的　对固定好的单卵裂球进行FISH检测 ,在最短的时间内获得清晰可靠的信号 ,进行临床分析。方法　选取发育不良的高风险胚胎分别进行分开变性和共变性后杂交的单细胞FISH ,统计并分析其信号结果。结果　共变性法信号获得率较分开变性法为高 ,并且信号获得率与变性方法相关 (P =0 .0 4 86 ) ;共有 9枚胚胎 ,4 2个卵裂球固定良好 ,其中二倍体信号 36个 ,单倍体信号 2个 ,2 1号染色体异常的胚胎为 4 9。结论　初探分析人IVF废弃胚胎 2 1号染色体异常几率较高。Objective: To detect samples as fast as possible, and gain clear and bright signals to analyse their clinical meanings. Methods: We do the experiments by separative-degeneration method and co-degeneration method, respectively, and analysed their clinical meanings after hybridization. Results: The co-degeneration method gained higher singal obtained rate, and the rate is correlated with degeneration method (P=0.0486). There are 42 nuclei of 9 embryos to be fixed well in total, and 38 of them had signals. The abnormal rate of the embryos is 4∶9. Conclusion: The abnormal rate of 21-chromosome is high in the human blastomeres.

关 键 词：荧光原位杂交技术 胚胎植入前遗传学诊断(PGD) 单细胞固定 21-三体 

分 类 号：R715[医药卫生—妇产科学]