单眼剥夺大鼠视觉系统三级神经元Bcl-2表达的研究  

Expression of the apoptosis inhibitory gene Bcl-2 in the neurons of visual system in monocularly deprived rats

作　　者：周凤[1] 邱良秀[2] 刘春民[1] 

机构地区：[1]暨南大学医学院附属深圳市眼科医院,深圳518001 [2]华中科技大学同济医学院附属同济医院眼科,武汉430022

出　　处：《眼科研究》2005年第1期26-29,共4页Chinese Ophthalmic Research

摘　　要：　目的　研究敏感期内和敏感期末单眼剥夺大鼠视觉系统三级神经元凋亡抑制基因Bcl 2表达规律及意义 方法　缝合 2周龄及 6周龄大鼠单侧眼睑 30d,切取视网膜中央区、外侧膝状体和视皮质 17区,行Bcl 2免疫细胞化学染色并对结果进行计算机图像分析。 结果　敏感期内剥夺眼较正常大鼠在视网膜节细胞层、同侧外侧膝状体相应输入层(A1层)和视皮质 17区(Ⅳ层)Bcl 2蛋白表达的免疫阳性神经元胞浆染色变淡(P<0 05), A1层、Ⅳ层的免疫阳性神经元数目减少(P<0 05),视网膜节细胞层免疫阳性神经元数目未见明显改变(P>0 05);敏感期末单眼剥夺大鼠视觉系统Bcl 2变化与对照组比较差异无显著性(P>0 05)。 结论　敏感期内单眼剥夺大鼠视觉系统视网膜节细胞层、外侧膝状体A1层和视皮质 17区Ⅳ层Bcl 2表达均降低,提示Bcl 2对视觉系统的作用可能是弱视发生的分子生物学基础之一。Objective To investigate the expression of the apoptosis inhibitory gene Bcl 2 in the neurons of visual system in monocularly deprived rats. Methods Unilateral eyelid suture was performed on 18 rats aged 2 week old rats and 6 week old rats for 30 days,and then the tissues of central retina,lateral geniculate nucleus(LGN) and visual cortex area 17 were taken for Bcl 2 staining by immunohistochemistry.The data were analyzed by optical microscopy and computer image system. Results In critical period,cytoplasm of Bcl 2 positive neurons in retinal ganglion cell layer,laminas receiving lateral geniculate nucleus (A1) and visual cortex area 17 (Ⅳ)were lighterly stained in comparison with those of the normal ground ( P <0 05),and the numbers of Bcl 2 positive neurons in the A1 and Ⅳ from deprived eyes were decreased ( P <0 05) and unchanged in ganglion cell layer ( P >0 05).There were insignificant difference in Bcl 2 expression between monocularly deprived rats and normal rats in the end of critical period ( P >0 05). Conclusion The expression of Bcl 2 gene was downregulated in retinal ganglion cell layer,the A1 layer of LGN and the Ⅳ layer of visual cortex area 17 in monocularly deprived rats during critical period,implying that Bcl 2 may play a role in the occurrence of amblyopia.

关 键 词：形觉剥夺 动物模型 视觉系统 BCL-2 

分 类 号：R777.44[医药卫生—眼科]