MMP-2mRNA、MMP-9mRNA在肺组织损伤修复中的表达  

The Expression of MMP-2mRNA AND MMP-9mRNA in Course of Injuries and Repairs of Lung Tissue

作　　者：唐四元[1] 周维[1] 唐发清[2] 

机构地区：[1]中南大学护理学院,湖南长沙410078 [2]中南大学湘雅医院

出　　处：《实用预防医学》2004年第6期1119-1121,共3页Practical Preventive Medicine

摘　　要：目的　观察MMP -2mRNA和MMP -9mRNA在肺组织损伤修复过程中的表达和变化规律 ,探讨它们在肺组织损伤修复中作用的分子机制和意义。　方法　 2 0只Wister大白鼠 ,随机分为一个正常组和臭氧攻击损伤后 8个时间的实验组 ,制作大鼠肺损伤模型。应用RT -PCR技术检测MMP -2、MMP -9基因表达规律。　结果　成功复制大鼠肺组织损伤动物模型 ;RT -PCR检测结果提示 :MMP -2、MMP -9基因在正常肺组织为弱表达 ,在肺组织损伤时表达下调 ,损伤修复开始时 ,表达又上调 ,且明显高于正常肺组织 ;当肺组织损伤修复达正常时 ,MMP -2、MMP -9基因表达水平减弱 ,接近正常水平。　结论　MMP -2mRNA、MMPObjective To explore the changes and expression of MMP-2mRNA and MMP-9mRNA in course of injuries and repairs of lung tissue, and their significance and probable molecular mechanism as well. Methods The research design is a time serial design. To establish the injury model of lung tissue in rats, 20 rats were divided into one control group and eight experimental groups receiving O 3 manipulation, RT-PCR for the gene expression regularity. Results The model of rats lung tissue injury was made successfully. RT-PCR showed MMP-2mRNA and MMP-9mRNA expressions were weak in normal lung tissue, and down-regulation expression in injurious lung tissue, and up-regulation expression in the lung tissue repairing and obvious higher than the normal lung tissue. The expression of MMP-2mRNA and MMP-9mRNA were weak when the lung tissue was repaired thoroughly. Conclusions The lower expressions of MMP-2mRNA and MMP-9mRNA were closely related to injury of lung tissue.

关 键 词：肺组织 基质金属蛋白酶 基因表达 损伤 

分 类 号：R-33[医药卫生]