甲基N-硝基亚硝基胍和视黄酸致ICR小鼠腭裂发育模型的建立  

Establishment of mouse model of cleft palate induced by N-methyl-N’-nitro-N-nitrosoguanidine and retinoic acid

作　　者：朱江波[1] 印木泉[1] 陈蓉芳[1] 郭苗莉[1] 张天宝[1] 

机构地区：[1]第二军医大学基础医学部卫生毒理学教研室,上海200433

出　　处：《第二军医大学学报》2005年第1期58-60,共3页Academic Journal of Second Military Medical University

基　　金：国家重点基础研究规划("973"计划)课题2901);国家自然科学基金(30070662;30400360)

摘　　要：目的:建立发生率较高、畸形类型明确、易于获得并可用于不同类型化学物致腭裂发生机制研究的动物模型。方法:采用致突变性化合物甲基N 硝基亚硝基胍(MNNG)和非致突变性致畸物视黄酸(RA)作用于ICR小鼠(对照组采用溶剂),观察不同剂量和不同给药时间的胚鼠腭裂畸形发生率,确定诱导腭裂发生的最佳作用条件,并通过光镜、电镜等手段对其畸形特征作进一步分析。结果:孕10 d一次给予40 mg/kg MNNG所致腭裂的发生率为55.2%,一次给予80 mg/kg RA腭裂发生率几乎达100%;光镜结果显示这2种化学物诱导的腭裂均为小腭;电镜结果表明孕16 d对照组腭中嵴上皮细胞表面有大量的丝状伪足而实验组中则无。结论:成功建立了可供两类不同腭裂发生分子机制研究的动物模型,为进一步研究腭裂畸形的分子机制奠定了基础。Objective:To develop a convenient,stable animal model of cleft palate with chemicals.Methods:N-methyl-N’-nitro-N-nitrosoguanidine(MNNG) and retinoic acid(RA) were used as teratogen to induce cleft palate in ICR mice.Morphological study was applied to analyze the cleft palate induced by MNNG and RA.Results:RA/MNNG-induced cleft palate of ICR mice were successfully established.It was showed that the palate shelves were reduced in size and lack of contact and fusion under microscope. The filopodia on surface of MEE cell was found in control group but not in treated group at TEM level.Conclusion:Animal models of 2 types of cleft palate induced by different chemical teratogens have been successfully established for studying the related molecular mechanism.

关 键 词：腭裂 动物模型 甲基N-硝基亚硝基胍 视黄酸 发育 

分 类 号：R782.22[医药卫生—口腔医学]