重症肌无力大鼠膈肌运动终板的形态学研究  

Research on morphological changes of end plates in diaphragm of experimental autoimmune myasthenia gravis rats

作　　者：刘睿[1] 李柱一[1] 张琪[2] 王桂平[1] 刘津平[3] 鞠躬[3] 

机构地区：[1]第四军医大学唐都医院神经内科,陕西西安710038 [2]北京武警总队医院口腔科,北京100039 [3]第四军医大学全军神经科学研究所,陕西西安710033

出　　处：《第四军医大学学报》2005年第2期112-114,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(30270485)

摘　　要：目的:探讨重症肌无力大鼠膈肌运动终板的光镜 及电镜结构变化.方法:被动转移乙酰胆碱受体单抗mAb35 建立SD大鼠获得性自身免疫性重症肌无力模型,用乙酰胆碱 酯酶法显示膈肌运动终板,并行超微结构观察.结果:实验组 大鼠注射mAb35后出现明显肌无力症状,其膈肌运动终板数 量基本不变,但出现与重症肌无力患者相似的超微结构变化, 对照组则无明显变化.结论:膈肌出现运动终板超微结构的 改变,是重症肌无力被动转移模型大鼠呼吸衰竭的可能原因.AIM: To study the pathological characteristics of the end plate on diaphragm in experimental autoimmune myasthenia gravis animal model by light microscopy and electron microscopy. METHODS: Passively transferred myasthenia gravis animal model was prepared by injection of acetylcholine receptor monoclonal antibody mAb35, and the ultrastructure of the end plates was studied by acetylcholinesterase histochemical technique. RESULTS: Myasthenia symptoms were induced in the experimental group. The end plates of diaphragm showed ultrastructural changes similar to those of myasthenia gravis patients but no change was found in the number of the end plates. No changes were observed in the control group. CONCLUSION: It is suggested that the ultrastructural changes of the end plates on diaphragm may result in the dyspnea in experimental autoimmune myasthenia gravis.

关 键 词：重症肌无力 乙酰胆碱酯酶 受体 胆碱能 运动神经肌肉终板 超微结构 

分 类 号：R746.1[医药卫生—神经病学与精神病学]