PKC拮抗剂对缩胆囊素刺激下的小鼠胰腺腺泡淀粉酶分泌的影响  

Effect of PKC antagonist-chelerythrine on mouse pancreatic secretion stimulated by cholecystokinin

作　　者：邓志波[1] 

机构地区：[1]汕头大学医学院第一附属医院,515031

出　　处：《中国实用内科杂志》2005年第1期53-54,共2页Chinese Journal of Practical Internal Medicine

摘　　要：目的　建立一个小鼠胰腺外分泌实验系统 ,用以研究促分泌物质缩胆囊素 (CCK)和卡巴胆碱 (CCH)对小鼠胰腺外分泌的影响 ,并使用细胞信号酶系统的蛋白激酶C(PKC)拮抗剂Chelerythrine以了解CCK及CCH的可能作用机制。方法　前瞻性对照性研究。结果　CCK和CCH均能刺激小鼠胰腺腺泡使其分泌淀粉酶比对照组增加约 1倍 (7 0 2 %比 14 2 9% ) ,但CCK或CCH加Chelerythrine则使淀粉酶的分泌增加大约 2倍 (19 0 2 % )。结论　CCK及CCH的刺激胰腺外分泌作用涉及到PKC信号系统 ,且PKC拮抗剂Chelerythrine有增强CCK及CCH的刺激作用。Objective To establish a mouse pancreatic exocrine secretion system,in order to study the effect of secretagogues,such as cholecystokinin (CCK) and carbachol (CCH),on the exocrine secretion of mouse pancreas,and use a protein kinase C (PKC) antagonist-chelerythrine(Che) to research the possible mechanism.Methods Prospective control study.Results CCK and CCH increased the secretion of amylase from the mouse pancreatic acini about two fold of that of the control (7.02%vs14.29%),and,CCK or CCH added Che,could increase the secretion of amylase to a level of about three fold(19.02%).Conclusion CCK or CCH stimulate the pancreatic secretion concerning the PKC cellular signal system;surprisingly,a PKC antagonist,chelerythrine,can reinforces their stimulation.

关 键 词：CCK 小鼠 PKC 拮抗剂 缩胆囊素 胰腺外分泌 刺激 CCH 增加 影响 

分 类 号：R735[医药卫生—肿瘤] F719[医药卫生—临床医学]