脑室内低温对脑局部缺血后神经元凋亡的影响  

Effects of hypothermia within the lateral ventricle on the neural cell apoptosis after rabbit cerebral ischemia

作　　者：吴伟[1] 史继新[1] 成惠林[1] 孙桂勤[2] 印红霞[1] 

机构地区：[1]南京军区南京总医院神经外科,江苏南京210002 [2]南京军区南京总医院病理科,江苏南京210002

出　　处：《医学研究生学报》2005年第1期20-22,F002,共4页Journal of Medical Postgraduates

摘　　要：目的 :研究兔侧脑室低温液滴注对脑局部缺血 再灌注损伤区神经元凋亡的影响。　方法 :眶入路以显微动脉瘤夹暂时夹闭左侧大脑中动脉 (MCA) 2h ,再灌注 2 4h ,制作脑局部缺血 再灌注模型。缺血后即刻 2 2℃无菌等渗盐水持续侧脑室滴注并引流 ,降低脑温至亚低温 (32～ 35℃ )后维持 2h ,再灌注 2 4h ,采用DNA片段原位末段标记技术 (TUNEL法 )分别检测对照组、夹闭组、低温组MCA供血区皮质神经元凋亡水平。　结果 :低温组缺血区凋亡神经元数明显低于夹闭组 (P <0 .0 1) ,而与对照组相比无明显差异 (P >0 .0 5 )。　结论 :脑室内低温方法可抑制脑缺血 再灌注后神经元凋亡 ,减轻脑缺血 再灌注的损伤。Objective: To explore the effects of continuous trickle of low temperature liquids through the lateral ventricle on neural cell apoptosis after rabbit local cerebral ischemia/reperfusion. Methods:The middle cerebral artery (MCA) of New Zealand rabbit was clipped by micro aneurysm clip for 2 hours and reperfused for 24 hours. Immediately after clipping the MCA, we trickled the left lateral ventricle continuously with low temperature liquids(22℃) to decrease the brain temperature to mild hypothermia (33℃-35℃)and maintained for 2 hours. After reperfusion for 24 hours , we assessed TUNEL method to determine the apoptotic cell rate in the sham-operated group, the control group and the mild hypothermia group respectively. Results:The apoptotic cell rate of the cortex tissues accommodated by MCA in the mild hypothermia group was obviously lower than that in the control group(P<0.01), but there was no obviously different comparing with the sham-operated group(P>0.05). Conclusion:Trickling ventricle with low temperature liquids could decrease the apoptotic cell rate and alleviate cerebral ischemia/reperfusion injury.

关 键 词：脑室 亚低温 脑缺血-再灌注 凋亡 

分 类 号：R743.31[医药卫生—神经病学与精神病学]