组织芯片技术检测食管癌前病变相关预警标志物  

Detection of tumor-associated biomarkers in precancerous lesions of esophagus

作　　者：滕梁红[1] 吕宁[1] 赵晓航[2] 薛丽燕[1] 刘芳[2] 林冬梅[1] 王卉心[2] 何祖根[1] 谢永强[1] 刘秀云[1] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所病理科,北京市100021 [2]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京市100021

出　　处：《世界华人消化杂志》2005年第1期6-9,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金;NO.30271460;No.30225045;No.30171049国家高技术(863)研究发展计划;No.2004AA227060

摘　　要：目的:检测annexin Ⅰ,Grp94,p53,Ki-67四种侯选肿瘤标志物在食管癌及癌前病变中的表达情况,为食管癌的早期检测提供依据. 方法:72例单纯手术切除食管鳞癌及其癌旁伴有的不同程度癌前病变,构建组织芯片;应用免疫组化方法检测annexin Ⅰ,Grp94,突变型p53,Ki-67蛋白在食管鳞癌及不同程度癌前病变中的表达差异. 结果:四种蛋白在食管癌前病变中均有异常表达,与正常鳞状上皮比较存在显著性差异:annexin Ⅰ在低度癌前病变组(轻度不典型增生/中度不典型增生)丢失率为68.3%,(P=0.000);p53和Ki-67的阳性表达率分别为67.6%和71.1%(P=0.000);Grp94在早期浸润性癌呈极显著过度表达,表达增强率62.0%(P=0.000). 结论:Annexin Ⅰ,Ki-67,p53蛋白为食管癌早期预警研究提供了重要线索;Grp94蛋白作为食管鳞癌进展期标志物具有参考价值.AIM: To investigate the expressions of annexin I, Grp94, p53, Ki-67 in esophageal squamous cell carcinoma (ESCC) and precancerous lesion, in order to provide valuable clues for early diagnosis. METHODS: Seventy-two specimens were collected, including dysplasia, in situ carcinoma, early ESCC, ESCC and lymph metastatic carcinoma. The expression of annexin I, Grp94, mutant p53 and ki-67 were detected using immunohistochemical staining (IHC). RESULTS: Compared with the normal esophageal epithelia, the expressions of annexin I, Grp94, mutant p53 and ki-67 were abnormal in precancerous lesions. Loss of annexin I (68.3%), positive expression of p53 (67.6%) and ki-67 (71.1%) were observed in low grade lesions (P<0.01), and Grp94 expression was increased significantly (62.0%) in early invasive cancer (P<0.01). CONCLUSION: Annexin I, mutant p53 and ki-67 may be the biomarkers of early ESCC, and Grp94 may be an important biomarker of progressive ESCC.

关 键 词：GRP94 食管癌前病变 食管鳞癌 标志物 KI-67 组织芯片技术 表达 结论 构建 参考价值 

分 类 号：R735.1[医药卫生—肿瘤] R737[医药卫生—临床医学]