雷公藤内酯醇诱导上皮性卵巢癌Caov-3凋亡的实验研究  

The Experiment Research of Apoptosis Induced By Triptolide on Ovarian Cancer

作　　者：李鹏[1] 孔北华[1] 刘秋燕[2] 郭振红[3] 张明徽[3] 江森[1] 

机构地区：[1]山东大学齐鲁医院,山东济南250012 [2]河北医科大学,河北石家庄050011 [3]上海第二军医大学免疫所,上海200433

出　　处：《实用妇产科杂志》2005年第1期24-27,共4页Journal of Practical Obstetrics and Gynecology

基　　金：国家自然科学基金资助 (30 2 71 361 )

摘　　要：目的 :观察雷公藤内酯醇对上皮性卵巢癌体外生长特性的影响 ,并进一步探讨有关的信号转导机制。方法 :分别采用MTT法、流式细胞仪测定和克隆形成实验观察雷公藤内酯醇对上皮性卵巢癌Caov 3增殖、凋亡和侵袭能力的影响 ,以Westernblot方法检测相关的信号分子。结果 :雷公藤内酯醇显著地抑制了Caov 3的增殖和克隆形成能力 (P <0 .0 5 ) ,诱导其凋亡 ,caspase途径和ERK活化主要参与了诱导凋亡的过程。结论 :雷公藤内酯醇对上皮性卵巢癌Caov 3生长特性的影响 ,使其有望成为一种新的化疗药物应用于卵巢癌的治疗。Objective: To observe the effect of Triptolide on the growth of epithelial ovarian cancer cell line and to investigate its signal transduction. Methods: The proliferative activities、Colony formation, and apoptosis-induced effect of triptolide on ovarian cancer cell line were measured by MTT assay, Colony formation assay, and flow cytometry, respectively. Signal transduction was detected by Western blot assay. Results: Triptolide inhibited the cell growth, Colony formation and induced apoptosis of ovarian cancer cell line CAVO-3 significantly (P<0.05). Conclusions: Triptolide had the potential to become a new kind of chemotherapy medicine.

关 键 词：雷公藤内酯醇 上皮性卵巢癌 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]