洛阳市GSTM1和GSTT1缺失基因型与肝癌的遗传易感性  

The null genotypes of GSTM1 and GSTT1 and the genetic susceptibility of primary liver cancer in Luoyang, China

作　　者：郭惠媛[1] 边建超[1] 江峰[1] 王启敏[2] 张竹梅[1] 樊巍巍[2] 王其军[2] 朱鑫[2] 汤伯明[2] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室,上海200032 [2]河南省洛阳市疾病预防控制中心流行病科

出　　处：《肿瘤》2005年第1期58-61,共4页Tumor

基　　金：国家自然科学基金资助项目 (编号:39870654)

摘　　要：目的探讨肝癌低发区谷胱甘肽转硫酶(GST)M1和T1的缺失基因型与肝癌的关系.方法应用多重PCR技术检测洛阳市95例肝癌患者和103例对照的GSTM1和GSTT1基因型.结果病例组GSTM1缺失基因型的频率为0.705,对照组为0.502,两者差异有显著性(x2=8.28,P=0.004),OR值为2.35(95%CI:1.25～4.41);病例组GSTT1缺失基因型的频率为0.611,对照组为0.437,两者差异有显著性(x2=5.97,P=0.015),OR值为2.02(95%CI:1.10～3.71).叉生分析表明该两因素在肝癌发生中有协同作用(x2=14.83,P=0.002),同时具有两个缺失基因型时,OR值为5.57(95%CI:2.03～15.66);GSTM1和GSTT1缺失基因型均与吸烟有协同作用,OR值分别为5.84(95%CI:2.26～15.47)和5.51(95%CI:2.13～14.54);GSTM1缺失基因型与饮酒有协同作用,OR值为3.31(95%CI:1.47～7.49),而GSTT1缺失基因型与饮酒无协同作用.结论在肝癌低发区GSTM1和GSTT1缺失基因型是肝癌的易感基因型.Objective To explore the association between the null genotypes of GSTM1 and GSTT1 and the genetic susceptibility of primary liver cancer(PLC) in the low-incidence area of PLC. Methods The GSTM1 and GSTT1 genotypes of 95 patients with PLC and 103 healthy controls were detected with the multiple polymerase chain reaction technique. Results The frequency of GSTM1 null genotypes is 0.705 in PLC patients, and 0.502 in controls. The difference is statistically significant(χ 2=8.28, P =0.004),and the odds ratio is 2.35(95% CI:1.25-4.41). The frequency of GSTT1 null genotypes is 0.611 in PLC patients, and 0.437 in controls. The difference is statistically significant(χ 2=5.97, P =0.015), and the odds ratio is 2.02(95% CI: 1.10- 3.71). The cross analysis showed a significant interaction between the two genotypes in the development of PLC(χ 2 =14.83, P =0.002),with an odds ratio of 5.57(95% CI:2.03-15.66). Smoking was interacted with both GSTM1 and GSTT1 null genotypes in the development of PLC, with the odds ratios of 5.84(95%CI:2.26-15.47) and 5.51(95%CI:2.13-14.54), respectively. The GSTM1 null genotype was interacted with alcohol drinking, with an odds ratio of 3.31(95%CI:1.47-7.49),and the GSTT1 null genotype was not interacted with alcohol drinking. Conclusion The GSTM1 and GSTT1 null genotypes are the susceptible genotypes of PLC in the low-incidence area of PLC.

关 键 词：肝肿瘤 谷胱甘肽转移酶类 基因型 吸烟 饮酒 

分 类 号：R735.1[医药卫生—肿瘤] R733.4[医药卫生—临床医学]