人肺腺癌细胞SPC-A1与人胚肺细胞WI-38对纳米颗粒吞噬作用的研究  

Study on endocytosis in vitro of aminosilane-coated magnetite nanoparticles by human lung adenocarcinoma cell line SPC-A1 and normal human lung embryo cell WI-38

作　　者：马勇杰[1] 李红[2] 许凯黎[2] 古宏晨[1] 

机构地区：[1]上海交通大学纳米科学与技术工程中心,上海200030 [2]上海交通大学肿瘤研究所,上海200032

出　　处：《肿瘤》2005年第1期7-9,共3页Tumor

基　　金：上海市纳米专项(编号:0249nm071);国家 863项目(编号:2002AA302210)

摘　　要：目的　探讨人肺癌细胞及胚肺细胞对氨基硅烷纳米Fe3O4颗粒的吞噬行为的生物学特性及二者之间吞噬特性的差异。方法　分别培养肺腺癌细胞株SPC A1及人胚肺细胞株WI 38至对数生长期,更换为分散有氨基硅烷纳米Fe3O4颗粒的培养液,经培养1、3、6h,分别取所培养的细胞进行超微结构观察。另取培养6h的部分SPC A1细胞传代培养,并对每一代细胞作超微结构观察。结果　培养1hr氨基硅烷纳米Fe3O4颗粒即已能进入癌细胞SPC A1的细胞质,培养至6h已见有大量颗粒成簇进入细胞质内。然而,在同一的条件下, 培养6h后仍未观察到氨基硅烷纳米Fe3O4颗粒进入WI-38细胞。经吞噬了氨基硅烷纳米Fe3O4颗粒的SPC A1肺癌细胞传至10代后仍生长良好,且在第7代仍然能观察到细胞内存在纳米颗粒并且未出现团聚现象。结论　在同一细胞培养的条件下,肺癌细胞SPC A1明显地比胚肺细胞WI 38更易吞噬氨基硅烷纳米Fe3O4颗粒,且前6h内未观察到纳米颗粒进入WI 38细胞。凡进入癌细胞内的纳米Fe3O4颗粒具有良好的生物相容性并在细胞中停留数代之久且保持良好的分散状态。根据本实验研究的结果进一步提示采用Fe3O4纳米颗粒将开拓一条颇有潜在性价值的纳米颗粒对肿瘤治疗的临床应用途径。Objective To study differential endocytosis of the aminosilane-coated magnetite particles between human lung adenocarcinoma cell line (SPC-A1) and normal human lung cell line (WI38) in vitro . Methods Both human lung adenocarcinoma cell line SPC-A1 and human lung embryo cells WI-38 were cultured respectively in medium contained aminosilane-coated nano Fe 3O 4 particles. From one to six hours, the ultrastructures of both cells were observed by transmission electron microscopy to determine particles uptake and their distribution in cells. Results Under the same conditions, human lung adenocarcinoma cell line SPC-A1 take up a lot of aminosilane- coated magnetite nanoparticles within the first 6 hours, while normal human lung embryo cells WI-38 do not take up any nanoparticles. Furthermore, one hour is enough for SPC-A1 to take up a few aminosilane-coated magnetite nanoparticles. The aminosilane-coated magnetite nanoparticles can still be observed intracellular SPC-A1 after 7 passages and keep a well-dispersed state. The subcultured cells of SPC-A1 taken up nanoparticles were in good condition after 10 passages. Conclusion The aminosilane-coated magnetite nanoparticles are taken up by SPC-A1 more easily than by WI-38 under the same conditions. Aminosilane-coated magnetite nanoparticles are well biocompatible and have low cytotoxicity. Since the nanoparticles could remain intracellular SPC-A1 for several generations and keep a well-dispersed state in the cytoplasm, it may be used to hyperthermia or chemotherapeutics on tumor for further clinical application .

关 键 词：肺肿瘤 细胞内摄作用 铁化合物 氨基硅烷 纳米颗粒 肿瘤细胞 培养的 

分 类 号：R734.2[医药卫生—肿瘤] O611.62[医药卫生—临床医学]