阿尔茨海默病患者红细胞电泳时间缩短  

The Reduction of the Time of Erythrocyte Electrophoresis in Alzheimer Disease

作　　者：张京立[1] 吴青[1] 陶国枢[1] 林娜[2] 季小莉[3] 赵莎莎 伊振宣 李爱国 张红红[1] 刘晓玲[1] 王鲁宁[1] 

机构地区：[1]解放军总医院老年医学研究所,北京100853 [2]军事科学院干休所门诊部,北京100091 [3]军事科学院门诊部,北京100091 [4]兵种部干休所门诊部,北京100039 [5]国防科工委第二干休所,北京100036 [6]总政黄寺干休所门诊部,北京100011

出　　处：《中国神经免疫学和神经病学杂志》2005年第1期18-20,共3页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家计委攻关基金资助项目(85 722 35 02)

摘　　要：目的　建立辅助诊断阿尔茨海默病(Alzheimer disease, AD)的实验室诊断方法。方法　采用 DextranT500和PEG8000混合液的下层液做电泳液。将50 V的恒压直流电加于长5. 6 cm、直径为1 5 mm的玻璃管两端,以1%(体积分数)的琼脂作盐桥。将肝素抗凝的 1 mL静脉血以 1 500 r/min离心 5 min,用磷酸缓冲液(PBS,pH6. 8)稀释100倍后,以1∶1的比例与下层液混合,充入电泳玻璃管。观察 AD组 (n=11)与对照组(n=38)红细胞泳动25μm的时间。结果　 AD组红细胞电泳时间为 (10 .95±2. 52)s, 对照组为(18 .74±4. 66)s, 两者差异具显著性(P<0. 001),两组数据交叉极少。结论　红细胞电泳时间缩短与 AD相关,对 AD具有一定辅助诊断价值。Objective To establish laboratory test method in diagnosis of Alzheimer disease (AD).Methods The lower layer of mixed solution from Dextran T 500 and PEG 8000 was used as the electrophoretic solution. The constant direct current of 50 V was added to the two ends of a glass tube which was 56 cm in length with a diameter of 15 mm through a pair of salt bridges of 1% agar-PBS(weight/volume ratio). 1 mL of anticoagulative venous blood was washed by PBS (pH68) at the condition of 1500 r/min, 5 min, and diluted to 1∶100 and mixed with the lower layer solution at the ratio of 1∶1. In the electrophoresis tube, the time of the electrophoresis of erythrocytes for 25 μm was determined. Results The erythrocyte electrophoresis time of Alzheimer patients[ (1095±252 )s, n =11]was significantly shorter than that of the control[ (1874±466 )s, n =38] , P<0001 The overlap of the data of the two groups was very little. Conclusions The reduction of the time of erythrocyte electrophoresis was corelated with AD. It seems to be an auxiliary tool for AD.

关 键 词：阿尔茨海默病 红细胞 诊断 

分 类 号：R741[医药卫生—神经病学与精神病学]