乳腺癌组织和血清中内皮细胞抑制素VEGF表达与肿瘤血管生成  

The Expression of VEGF and Endostatin Both in Tissues and Sera and the Angiogenesis of Breast Cancer

作　　者：赵建华[1] 唐金海[2] 孟凡青[3] 秦建伟[2] 赵祥生[2] 

机构地区：[1]江苏省肿瘤医院江苏省临床检验中心,南京市210009 [2]江苏省肿瘤医院普外科,南京市210009 [3]南京大学医学院附属鼓楼医院病理科

出　　处：《中国肿瘤临床》2005年第2期79-82,共4页Chinese Journal of Clinical Oncology

基　　金：江苏省科技厅社会发展基金资助(编号:BS99437)

摘　　要：目的:研究乳腺癌患者癌组织和血清内皮细胞抑制素(ES)、VEGF表达和肿瘤血管生成间的关系,初步探讨乳腺癌血管生成调节机制。方法:采用免疫组化法检测59例乳腺癌组织ES、VEGF表达及其微血管密度(MVC);分别采用竞争性酶联免疫试验和ELISA测定患者乳腺癌组织和手术前、后血清ES及VEGF水平。结果:1)癌组织ES、VEGF阳性表达率分别为69.5%和59.3%,明显高于癌旁组织(P<0.005)。2)手术前乳腺癌组血清ES和VEGF水平显著增高;手术3周后,血清VEGF水平明显下降,而ES仍保持在较高水平。3)癌组织VEGF表达、组织和血清VEGF水平间具有密切相关性(P<0.05);癌组织ES表达和血清ES水平间仅有弱的关联(P=0.06)。4)癌组织VEGF阳性和阴性组,MVC分别为37.5±10.3和22.8±8.3,有显著性差异(P<0.001),且血清VEGF浓度与MVC呈正相关(P<0.0001,r=0.63);癌组织VEGF表达阴性组,血清ES与MVC显示负相关(P=0.03,r=-0.45)。结论:VEGF是促进乳腺癌血管生成的直接相关因子,而ES可能是肿瘤及其转移的血管生成负调控因子。Objective: To investigate the correlations among the angiogenesis of breast cancer and the expressions of endostatin (ES) and VEGF both in tissues and sera, and to discuss the potential tumor angiogenesis regulatory mechanism. Methods: The expressions of VEGF, ES and CD34 in 59 cases of patients with breast cancer were analyzed by immunochemistry. The VEGF and ES levels in tissues and sera before and after operation were determined using competitive enzyme immunoassays and ELISA, respectively. Results: 1) Positive expression rates of ES and VEGF in tumor tissues were 69.5% and 59.3% respectively, significantly higher than those in surrounding tissues (P<0.005). 2) Before operation, the level of ES and VEGF in serum was significantly high; after operation, the VEGF level decreased significantly and the ES level remained at a high level. 3) There was the direct correlations between VEGF expression, VEGF concentration in tissue extracts or serum VEGF level (P<0.05). A weak correlation between the positive ES expression and high serum ES level was existed (P=0.06). 4) MVC in cases with the positive VEGF expression was significantly higher than that with the negative VEGF (P<0.001), and the serum VEGF level was positively related to MVC (P<0.0001, r=0.63). Serum ES level in the cases with negative VEGF expression showed a negative correlation with MVC(P=0.03, r=-0.45). Conclusion: In breast cancer, VEGF may directly contribute to the promotion of angiogenesis, and ES might inhibit tumor angiogenesis and metastases.

关 键 词：血管生成 乳腺癌 内皮细胞抑制素 VEGF 

分 类 号：R737.9[医药卫生—肿瘤]