MTT法检测AU_(14-1)介导LAK细胞的细胞毒效应  

MTT Colorimetric Assay for Cytotoxic Activity of AU_(14-1) mediated LAK

作　　者：王红梅[1] 徐方云[1] 曾小平[2] 邬淑云[1] 温淦升[1] 

机构地区：[1]江西医学院病理生理学教研室,肿瘤免疫研究室,江西南昌330006 [2]江西医学院免疫学教研室,江西南昌330006

出　　处：《江西医学院学报》2004年第6期29-30,34,F002,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的　应用MTT比色法研究抗小鼠宫颈癌单克隆抗体 (McAb)AU14 1介导淋巴因子激活的杀伤细胞 (lym phokineactivatedkillercells,LAK)对靶肿瘤细胞的体外细胞毒作用。 方法　通过检测不同数量小鼠宫颈癌U14 细胞与加入四甲基偶氮唑盐 { [3 (4,5 dimethylthiazol 2 yl) 2 ,5 diphenylterazoliumbromide],MTT}后形成甲颗粒所产生的不同吸光度 (A)值 ,建立测定的线性关系。进而应用MTT比色法研究McAbAU14 1介导LAK细胞的体外细胞毒作用。结果　当U14 细胞在 10～ 10 5个 /孔以内时 ,活细胞数与A值呈直线相关 ;LAK细胞对于经AU14 1处理的肿瘤细胞的杀伤率显著高于未经AU14 1处理的肿瘤细胞 (P <0 .0 1)。结论　McAbAU14 1可加强LAK细胞对靶细胞的溶解作用。提示特异性宫颈癌单克隆抗体AU14 1介导的细胞毒在治疗宫颈癌中可能是一种有潜在价值的方法。Objective To investigate the cytotoxicity of McAb A U 14-1 mediated LAK against target cervical carcinoma by MTT colorimetric assay.Methods MTT assay was employed to study the relationship betw een A value and different numbers of viable U 14 cells with the aim to esta blish the standard linearity with which the cytotoxic activities of McAb AU 1 4-1 mediated LAK in vitro were evaluated.Results It revealed a l inearity between the different numbers of the cells and A value; The cytotoxicit y mediated by LAK to U 14 cells treated with McAb AU 14-1 was signifi cantly higher than those not treated with it.Conclusion McAb AU 14-1 could enhance the cytotoxicity of LAK to U 14 cells,suggesting its ability to direct ADCC make this antibody promising candidate for cervical cance r therapy.

关 键 词：MTT比色法 LAK细胞 抗体依赖细胞介导的细胞毒 

分 类 号：R-332[医药卫生]