急性重症胆管炎致急性肺损伤动物模型的制作  

Prepation of acute lung injurg induced acute severe cholangitis

作　　者：崔华雷[1] 秦明放[2] 鲁焕章[2] 卢奕[3] 姚智[3] 曹玉梅[4] 刘敬[5] 

机构地区：[1]天津市儿童医院 [2]天津市南开医院 [3]天津医科大学 [4]天津市河东中心医院 [5]天津师范大学生物系

出　　处：《天津医科大学学报》2000年第4期410-412,共3页Journal of Tianjin Medical University

基　　金：天津市自然科学基金资助 !(941309012)

摘　　要：目的 :复制急性重症胆管炎(ACST)引发的急性肺损伤 (ALI)的动物模型。方法 :通过胆总管远端结扎 ,近端注入菌液并封闭的方法 ,造成大鼠ACST后观察肺功能及病理形态改变。结果 :实验组PaO2/FiO2 下降 ;肺系数升高 ,肺含水量增加 ;光镜可见充血、水肿、粒细胞浸润和透明膜。结论 :此模型符合ALI诊断标准及动物模型的考察指标 。Objective: To make the animal model of acute cholangitis of severe type (ACST) resulting in ALI. Methods: In this study, the distally ligating and proximally injecting bacteria liquid at choledochus caused ACST in rats, after which the functional and morphologic change was observed. Results: The results of the model group were as follow: 1. PaO2 /FiO2 decreased. 2.The index of lung was upward and the water content in the lung were increased. 3.The congestion, edema, granular infiltration and hyaline membrane were found under the optical microscope. Conclusion: The model was up to diagnostic standard and observation index of ALI, so we regarded that this model preparation was successful.

关 键 词：急性重症胆管炎 动物模型 急性肺损伤 

分 类 号：R575.7[医药卫生—消化系统]