低氧诱导因子-1α和环氧合酶-2蛋白在炎症性肠病中的表达  

Expression of hypoxia-inducible factor-1 alpha and cyclooxygenase-2 protein in inflammatory bowel disease

作　　者：许春梅[1] 董卫国[1] 余保平[1] 罗和生[1] 于皆平[1] 

机构地区：[1]武汉大学人民医院消化内科,430060

出　　处：《临床内科杂志》2005年第2期82-84,共3页Journal of Clinical Internal Medicine

基　　金：武汉市科技局项目资助(NO:301121017)

摘　　要：目的　探讨低氧诱导因子 1α(HIF 1α)和环氧合酶 2(COX 2)蛋白在活动性炎症性 肠病中的表达及其作用。方法　采用兔抗人HIF 1α、COX 2蛋白抗体和枸橼酸 微波 SP 免疫组织 化学方法检测60例甲醛固定、石蜡包埋的活动性炎症性肠病活检蜡块标本以及10例正常结肠黏 膜组织中HIF 1α和COX 2蛋白表达情况。结果　HIF 1α蛋白在47例溃疡性结肠炎、13例克隆氏 病、10例正常结肠黏膜组织中的细胞表达阳性率分别为57.07%±13.83%、59.27%±8.59%和 2.21%±0.46%,前两者分别与后者比较差异有显著性(P<0.01),而前两者之间比较差异无显著 性(P>0.05);COX 2蛋白在47例溃疡性结肠炎、13例克隆氏病、10例正常结肠黏膜组织中的细 胞表达阳性率分别为56.74%±9.67%、59.34%±4.12%和1.89%±0.36%,前两者分别与后者 比较差异有显著性(P<0.01),而前两者之间比较差异无显著性(P>0.05);HIF 1α和COX 2蛋白 水平呈正相关(溃疡性结肠炎:r=0.690;克隆氏病:r=0.723),P<0.05。结论　HIF 1α和COX 2 蛋白均参与了活动性炎症性肠病的发病,HIF 1α可能是COX 2基因表达中的一个重要调节者。Objective To investigate the expression and action of hypoxia-inducible factor-1 alpha(HIF-1α) and cyclooxygenase-2 (COX-2) protein in active inflammatory bowel disease.Methods The expressions of HIF-1α and COX-2 protein in formalin-fixed,paraffin-embedded colon tissues from 60 cases with inflammatory bowel disease and 10 healthy controls were detected by citrate-microwave-streptavidin-HRP(SP) immunohistochemical method.Results The positive rates of HIF-1α expression were 57.07%±13.83%,59.27%±8.59%,2.21%±0.46% and those of COX-2 expression were 56.74%±9.67%,59.34%±4.12%,1.89%±0.36% in 47 ulcerative colitis,13 Crohn’s disease,10 normal colon mucosa respectively.A marked difference of HIF-1αand COX-2 expression was seen between ulcerative colitis and normal colon mucosa, and the same as between Crohn’s disease and normal colon mucosa(P<0.01).But the no difference was detected between ulcerative colitis and Crohn’s disease (P>0.05).Linear correlation analysis showed that COX-2 protein was positively correlated with HIF-1α protein(ulcerative colitis:r=0.690;Crohn’s disease:r=0.723,P<0.05).Conclusion HIF-1αand COX-2 are involved in the pathogenesis of active inflammatory bowel disease,HIF-1α may be an essential regulator of COX-2 gene expression.

关 键 词：炎症性肠病 低氧诱导因子-1Α 环氧合酶-2 免疫组织化学 

分 类 号：R574.62[医药卫生—消化系统]