飞蓬属多种植物的化学成分含量研究  

Determination Flavonoids and Scutellarin in Herbs of Erigeron genus

作　　者：胡宇慧[1] 张浩[1] 张志锋[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《药物分析杂志》2005年第1期21-23,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家教育部博士点基金(20020610089)

摘　　要：目的:比较飞蓬属五种植物中总黄酮及灯盏乙素(scutellarin)的含量;建立灯盏乙素的RP-HPLC测定法。方法:以芦丁为对照品,比色法测定总黄酮的含量;采用ODS柱,甲醇-水-冰醋酸(38:62:0.8)为流动相,检测波长335nm,测定灯盏乙素的含量。结果:四川产的多舌飞蓬中总黄酮含量最高,四川产的飞蓬中灯盏乙素含量最高。结论:所建立的灯盏乙素的RP-HPLC测定法准确,简便,灵敏度高,重复性好,可以用于飞蓬属多种植物中的灯盏乙素的含量测定。同时为我们进一步开发飞蓬属植物提供了理论依据。Objective:Tocompare with the content of total flavonoids and scutellarin, and to establish a method to determine scutellarin by RP-HPLC.Methods:To take Rutin as reference,and determined total flavonoids by vis colorimetric analysis;The sample was separated on an ODS- 18 column,The mobile phase was methanol -waterice acetic acid(38:62:0.8).The determination wavelength was 335nm.Results:total flavonoids was the highest in Erigeron multiradiatus, and the content of scutillarin in Erigeron acer was higher than others.Conclusions:The methods were precise, convenient, reproducible for determination of scutellarin in Erigeron genus.At the same time, it give us some scientific information for developing them further.

关 键 词：灯盏乙素 含量 RP-HPLC测定 总黄酮 对照品 多舌飞蓬 化学成分 种植 植物 流动相 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]