香蕉束顶病毒海南分离物DNA组分的克隆及序列分析  

Cloning and Sequencing of DNA Components of Banana bunchy top virus Hainan Isolate

作　　者：田娥[1] 庄军[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101

出　　处：《农业生物技术学报》2004年第6期680-684,共5页Journal of Agricultural Biotechnology

摘　　要：以中国海南岛地区表现束顶病毒(Banana bunchy top virus, BBTV)症状的香蕉组织总DNA为模板,通过PCR方法克隆了BBTV海南分离物的6个DNA组分全序列,并进行了核苷酸序列分析。测序结果表明,DNA1～6序列全长分别为1104、1067、1059、1045、1014和1081 nt,均已登录到GenBank(登录号分别为AY450396、AY606084、AY494786、AY494788、AY606085和AY494787)。与世界不同地域分离物进行序列同源分析,发现DNA1序列较保守,与越南分离物的同源性达到了95.4％;而DNA2和DNA4变异较大,其中DNA2序列与广州分离物的同源性仅有83％。根据DNA1的序列差异分析初步确定BBTV海南分离物属于亚洲亚组。Six DNA components of Banana bunchy top virus (BBTV) were amplified by PCR using total DNA from banana tis- sues which arc of characteristic of typical BBTV symptom as template. The sequence analysis indicated that the 6 fragments were all full-length components of BBTV, they were 1104, 1067, 1059, 1045, 1014 and 1081 nt respectively. All of them have been sub- mitted to GenBank(Accession No. AY450396, AY606084, AY494786, AY494788, AY606085 and AY494787, respectively). The vi- ral sequences from different countries were compared. Results revealed that the sequence of component 1 was more conservative than other components, DNA1 of Hainan isolate shared homologies of 95.4% with that of Vietnam isolate, while both the sequences of component 2 and component 4 had more difference, and DNA2 of Hainan isolate only shared homologies of 83% with that of Guangzhou isolate. According to DNA1 sequence comparison results, the BBTV Hainan isolate should belong to Asia sub-group.

关 键 词：分离物 束顶病 香蕉 总DNA 同源性 核苷酸序列分析 病毒 克隆 海南 序列差异分析 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治] S435[农业科学—植物保护]