反相高效液相色谱法测定一株新分离的两歧双歧杆菌DNA G+C摩尔百分含量  

Determination of the G+C mol% of DNA in a Novel Strain of Bifidobacterium bifidum by Reversed-Phase High Performance Liquid Chromatogrephy

作　　者：吴芸[1] 李惠芝[1] 马永平[2] 邱宗荫[1] 

机构地区：[1]重庆医科大学药学系,重庆400016 [2]重庆医科大学基础医学院,重庆400016

出　　处：《西南师范大学学报（自然科学版）》2005年第1期96-100,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30200244).

摘　　要：建立了用反相高效液相色谱法测两歧双歧杆菌 DNA G+ C 摩尔百分含量的方法. 采用 20 mmol·L-1KH2PO4(pH 5 6)缓冲液—甲醇(体积比90∶10)为流动相, 检测波长260 nm, 流速1 mL·min-1, 在Kromasil C18柱(5μm, 250 mm×4.6 mm i d )上对各碱基进行分离, 以峰面积计算, 计算值和文献值相符.A method was established to determine the G+C mol% of DNA in Bifidobacterium bifidum. Various bases were separated on a Kromasil C_(18) (5 μm, 250 mm×4. 6mm i. d. ) column by using KH_2PO_4 buffer (20 mmol·L^(-1), pH 5. 6)-methanol(90∶10) as mobile phase at a flow rate of 1 mL·min^(-1). The UV detection was set at 260 nm. The calculative value was identical with the documental value after the calculation by making use of peak areas.

关 键 词：两歧双歧杆菌 G+C摩尔百分含量 反相高效液相色谱 

分 类 号：O657.7[理学—分析化学] Q503[理学—化学]