转染野生型p53基因对人肝癌细胞生长的抑制作用  

Growing inhibition of hepatocarcinoma cells by transfection with wild-type p53 gene

作　　者：赖祥进[1] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,南宁530021

出　　处：《广西医学》2005年第2期165-167,共3页Guangxi Medical Journal

基　　金：广西壮族自治区自然科学基金资助项目 (桂科自 9912 0 35 )

摘　　要：目的　研究转染野生型 p5 3基因对人肝癌细胞的生长影响作用。 方法　将克隆有野生型 p5 3基因的 pUHD10 3质粒 ,通过Lipofectin转染肝癌细胞株BEL 74 0 2、BEL 74 0 4、HLE、HuH 7及SMMC 772 1,用四甲基偶氮唑 (MTT)法观察细胞的生长情况。结果　转染野生型p5 3基因 ,可使肝癌细胞BEL 74 0 4、HLE、HuH 7的生长明显受到抑制 ,第 4天生长抑制率分别是5 0 .0 %、6 9.0 %及 6 5 .1%。结论　转染野生型 p5 3基因能有效抑制肝癌细胞株BEL 74 0 4、HLE、HuHObjective To observe the growing effection in hepatocarcinoma cells of transfection with wild-type p53(wt-p53)gene.Methods The hepatocarcinoma cell line BEL-7402,BEL-7404,HLE ,HuH-7 and SMMC-7721 were transfected by the Plasmids pUHD10-3 recombined with wild-type P53 gene at lipofectin-mediated. The growing tests of the hepatocarcinoma cells were studied by monotetrazolium(MTT) analysis.Results The growth of the hepatocarcinoma cell line BEL-7404,HLE as well as HuH-7 were observably suppressed after transfection with the wt-p53 gene.Conclusion The wt-p53 gene effectively suppressed the growth of hepatocarcinoma cell line BEL-7404,HLE and HuH-7.

关 键 词：P53基因 肝癌 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] Q785[医药卫生—临床医学]