P27在人皮肤血管瘤不同时期的表达及意义  

Expression of P27 at Different Stage of Human Dermal Hemangiomas

作　　者：沈鹏[1] 陕声国[1] 张端莲[2] 余瑛[2] 

机构地区：[1]武汉大学人民医院整形外科,武汉430060 [2]武汉大学医学院人体解剖学与组织胚胎学系,武汉430071

出　　处：《武汉大学学报（医学版）》2005年第1期42-44,47,i002,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目 (批准号 :3 0 2 713 45 )

摘　　要：目的 :探讨P2 7蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法 :采用免疫组织化学方法 (SP法 )检测人皮肤血管瘤增生期、退化期及正常皮肤组织中P2 7的表达水平 ,并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达P2 7的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织P2 7表达的平均光密度和平均阳性面积率。结果 :增生期血管瘤内皮细胞P2 7表达水平低于退化期 ,差异有显著性 (P <0 .0 0 5 ) ,退化期血管瘤内皮细胞P2 7表达水平与正常皮肤组织相比 ,差异有显著性 (P <0 .0 0 1)。结论 :P2 7通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。Objective: To investigate the expression and significance of P27 in the occurrence, development and regression of human hemangiomas.Methods: The expression of P27 was examined in proliferating, involuting human hemangiomas and normal skin tissues by using immunohistochemical technique. Immunohistochemical technique for factor Ⅷ-related antigen was used to prove that the cells which expressed P27 were endothelium.Image analysis system was applied to measure the expressing level of P27 at different stage of hemangiomas and in normal skin tissues. Results: The expression of P27 was significantly lower in proliferating hemangiomas than in involuting hemangomas (P<0.005),and was significantly lower in the involuting hemangiomas than in normal skin tissues. (P<0.001). Conclusion: It is suggested that P27 might play an important role in the regression of human hemangiomas endothelial cells and anti-angiogenesis.

关 键 词：血管瘤 P27 内皮细胞 血管生成 

分 类 号：R732.2[医药卫生—肿瘤]