卵磷脂中PE及不同种类PC的高效液相色谱分析  

Analysis of phosphatidylethanolatimine and different phosphatidylcholines in the lecithin by high performance liquid chromatography

作　　者：刘静[1] 宋航[1] 付超[1] 黄明刚[1] 

机构地区：[1]四川大学化工学院,四川成都610065

出　　处：《化学研究与应用》2005年第1期134-136,共3页Chemical Research and Application

摘　　要：Phosphatidylcholine(PC)and phosphatidylethanolatmine(PE)are successfully separated,meanwhile various components of phosphatidylcholines can also be separated by using the following operating conditions:the Lichrosorb Si 60 column(4.6mm×250mmi.d.5μm),hexane:iso-propanol:water:85%phosphate(45:48:7.5:0.15,volume ratio)as mobile phase,40℃,1.0mL/min flow rate and UV detection at 206 nm.This method is very simple,and its recovery and accuracy are very high,so it is especially suitable for the preliminary analysis of phosphatidylcholine components.Phosphatidylcholine(PC)and phosphatidylethanolatmine(PE)are successfully separated,meanwhile various components of phosphatidylcholines can also be separated by using the following operating conditions:the Lichrosorb Si 60 column(4.6mm×250mmi.d.5μm),hexane:iso-propanol:water:85%phosphate(45:48:7.5:0.15,volume ratio)as mobile phase,40℃,1.0mL/min flow rate and UV detection at 206 nm.This method is very simple,and its recovery and accuracy are very high,so it is especially suitable for the preliminary analysis of phosphatidylcholine components.

关 键 词：高效液相色谱 磷脂酰胆碱(PC) 磷脂酰乙醇胺(PE) 卵磷脂 

分 类 号：O657.72[理学—分析化学]