复合氨基酸胶囊中水溶性维生素的含量测定  

Assay of the Water-soluble Vitamins in the Compound Amino Acid Capsules

作　　者：王曼熙 

机构地区：[1]深圳万和制药有限公司,深圳518055

出　　处：《中国药品标准》2004年第6期28-30,共3页Drug Standards of China

摘　　要：目的:建立复合氨基酸胶囊中5种水溶性维生素的含量测定方法。方法:采用高效液相色谱法,用乙腈与庚烷磺酸钠-磷酸钠的水溶液(pH=3.0)为流动相,流速1.0ml·min^(-1),色谱柱为 C_(18)(4.6×250mm,5μm),柱温40℃。检测波长为280nm,进样量为20μl。结果:维生素 B_1、维生素 B_2、维生素 B_6、维生素 C 和烟酰胺分别在24.7～75.2μg·ml^(-1)、15.0～44.9μg·ml^(-1)、12.2～37.5μg·ml^(-1)、99.2～299.9μg·ml^(-1)和100.3～300.9μg·ml^(-1)范围内呈线性关系,相关系数均为1.000,平均回收率均在100%±2%范围以内。RSD 分别为0.19%、0.26%、0.46%、1.17%和0.52%。结论:本方法准确,简便,快捷,可以同时测定复合氨基酸胶囊中维生素 B_1、维生素 B_2、维生素 B_6、维生素 C 和烟酰胺的含量。Objective:To establish a method for assaying five water-soluble Vitamins in the Compound Amino Acid Capsules. Method:With the HPLC method,mobile phase was acetonitrile and 1.0mol·1^(-1).Column was C_(18)(4.6×250mm 5μm)and was maintained at a constant temperature of about 40℃.The detective wavelength was 280nm.The injection equal volume was 20μl.Results:The standard curve of VitB_1 was linear in the concentration range of 24.7～75.2μg·ml^(-1),VitB_2 was linear in the concentration range of 15.0～44.9μg·ml^(-1),VitB_6 was linear in the concentration range of 12.2～37.5μg·ml^(-1),VitC was linear in the concentration range of 99.2～299.9μg·ml^(-1)and Nicotinamide was linear in the concentration range of 100.3～300.9μg·ml^(-1),the correlation coefficient was 1.000.The average recovery rate of VitB_1,VitB_2,VitB_6,VitC and Nicotinamide was in the range of 100%±2%,RSD was 0.19%、0.26%、0.46%、1.17% and 0.52%,respectively.Conclu- sions:This method is found to be accurate,simple and quick.It can simultaneously assay for VitB_1,VitB_2,VitB_6,VitC and Nicotinamide in Compound Amino Acid Capsules.

关 键 词：胶囊 水溶性维生素 含量测定 维生素B1 维生素B2 维生素C 烟酰胺 复合氨基酸 维生素B5 磺酸 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]