恒河猴感染SARS病毒致病模型的建立  

Establishment of Pathogenicity Model of Severe Acute Respiratory Syndrome Virus Infection on Macaca Rhesus

作　　者：侯炜[1] 杨占秋[1] 朱润庆[2] 魏文进[3] 周俊[1] 钟琼[1] 王薇[1] 唐志佼[2] 汤宏斌[3] 鲜巧阳[3] 王勇[3] 文利[1] 

机构地区：[1]教育部病毒学重点实验室/武汉大学医学院病毒学研究所,湖北武汉430071 [2]武汉大学医学院病理生理系,湖北武汉430071 [3]武汉大学实验动物中心,湖北武汉430071

出　　处：《中国病毒学》2004年第6期545-548,i009,共5页Virologica Sinica

基　　金：中国医学基金会新药基金;北京华特森基因科技有限公司资助项目

摘　　要：为建立恒河猴严重急性呼吸道综合征(SARS)的模型并对其致病特点进行观察,采用病毒分离、免疫荧光、光镜及 RT-PCR 方法对病毒感染组和非感染组恒河猴不同时间、不同组织或分泌物进行检测。结果显示从恒河猴不同组织中分离到病毒,而且在病毒感染后第 2d 和第 5d 的血液、第 7、9d 的鼻咽分泌物、第 3d 的粪、第 5d 的粪尿中均检测到 SARS-CoV RNA。光镜观察到病毒感染组肺组织肺泡间隔增宽,有大量淋巴细胞、单核细胞浸润,肺泡腔有渗出,甚至形成透明膜样物;多个肺泡形成机化性肺炎的表现。感染组肝组织可见较大的坏死灶,并伴有大量炎性细胞浸润。结论认为已成功建立了恒河猴 SARS 模型,可用于评价抗 SARS 药物和疫苗的研究。To create the pathogenicity model of severe acute respiratory syndrome (SARS) on macaca rhesus, the macacu rhesus was infected with SARS coronavirus (SARS CoV) was detected in different samples coming from macaca rhesue in different days by virus isolation, immunoflurescence assay, pathological inspection and reverse transcription polymerase chain reaction (RT-PCR). The results showed that SARS CoV were isolated from the above samples, and SARS CoV RNA could be detected in blood of the 2nd and 5th day, secreta of nose-throat of the 7th and 9th day, faeces of the 3th day, and faeces and urines of the 5th day after infection. Under the microscope, it was found in the group infected by SARS CoV that pulmonary alveolus interval had been broadened and had many lymphocytes and monocytes infiltrated. There was much exudation in the chamber pulmonary alveolus, which even formed the hyaline membrane. The organized pneumonia could be found in several pulmonary alveolus. great necrosis foci was also observed in the liver, companied by some inflammatory cells infiltrated. It may be successful to create the model of SARS on macaca rhesus, and show that the animal model can be used to evaluate anti-SARS CoV drugs and vaccines.

关 键 词：严重急性呼吸道综合征(SARS) 致病性 模型 

分 类 号：R-332[医药卫生]