模拟胆汁体系中胆固醇结石内细菌对胆固醇晶体形成的作用  

The study of the pro-nucleating activity of bacteria identified in cholesterol gallstones in model bile systems

作　　者：朱雷明[1] 蔡端[1] 吕元[2] 陈为欢[2] 王文风[3] 张延龄[1] 

机构地区：[1]复旦大学附属华山医院外科,上海200040 [2]复旦大学附属华山医院检验科,上海200040 [3]复旦大学医学院微生物教研室

出　　处：《中华外科杂志》2004年第24期1501-1504,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目(30070737)

摘　　要：目的探讨胆固醇结石中的细菌与结石形成的关系。方法观察胆固醇结石中的细菌在模拟胆汁中的生长活性及其对胆固醇晶体成核时间(NT)的影响。结果(1)模拟胆汁中大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌、产孢梭菌和艰难梭菌生长旺盛;痤疮丙酸杆菌长势偏弱;脆弱类杆菌生长受抑制。(2)仅铜绿假单胞菌和粪肠球菌能缩短胆固醇晶体成核时间。(3)加入铜绿假单胞菌或粪肠球菌的胆汁,晶体形成呈现缓慢的阶段性演变过程。结论铜绿假单胞菌和粪肠球菌在模拟胆汁中具有促成核活性,而痤疮丙酸杆菌无促成核作用。Objective To explore the relationship of bacteria identified in cholesterol gallstones and gallstone formation. Methods Observe the bacteria activity in model bile and the influence of bacteria on the cholesterol nucleation time (NT). Results (1) Model bile were suitable for the growth of E. coli, Pseudomonas aeruginosa, staphylococcus aureus, enTerococcus faecalis, clostridium difficile and Clostridium sporegenes. Propionibacterium acne grew weakly and the growth of Bacteroides fragilis was restrained in model bile. (2) Only pseudomonas aeruginosa and enTerococcus faecalis could ly shorten the cholesterol nucleation time. (3 ) With pseudomonas aeruginosa or enTerococcus faecalis added in model bile, the formation of cholesterol crystals presented a progressive course of evolution. Conclusions Pseudomonas aeruginosa and enTerococcus faecalis, not propionibacterium acne, have pro-nucleating ability in model bile.

关 键 词：胆汁 胆固醇结石 胆固醇晶体 铜绿假单胞菌 粪肠球菌 痤疮丙酸杆菌 细菌 体形 产孢 成核 

分 类 号：R575.62[医药卫生—消化系统]