人类XRCC_1-399单核苷酸多态性与原发性肝细胞癌的相关研究  

Study on the association of human XRCC_1-399 single nucleotide polymorphism and primary hepatocytic carcinoma

作　　者：韩永年[1] 杨金龙[1] 郑水根[1] 吴一迁[2] 

机构地区：[1]上海市第八人民医院,200235 [2]上海市肿瘤研究所

出　　处：《肝脏》2004年第4期235-237,共3页Chinese Hepatology

摘　　要：目的　以病例 对照研究方式探讨人类DNA修复基因XRCC1-3 99单核苷酸多态性 (SNP)与HBV感染者的原发性肝细胞癌 (HCC)的发生关系。方法　 72例HCC患者经病理检查证实 ,根据地缘、性别、年龄 ,按 1∶1～ 2比例匹配 13 7例非HCC对照者。采用聚合酶链反应 限制片段长度多态性 (PCR-RFLP)技术检测受试者XRCC1-3 99位SNP。结果　 (1)XRCC1-3 99SNP和年龄均与HCC的发生无关 ,但在XRCC1 3 99Arg/Arg受试者中 ,HCC的发生与年龄呈负相关 (P =0 .0 2 8) ;(2 )HBV感染是HCC发生的肯定因素 (P =0 .0 0 7) ;在XRCC1 3 99Gln/Gln或Arg/Gln受试者中 ,伴HBV感染者HCC发生率 (2 5 .7% )远高于不伴HBV感染者 (5 .3 % ,P =0 .0 47) ;(3 )XRCC1 3 99Arg/Arg受试者HBV感染率与Gln/Gln或Arg/Gln受试者近似 (3 6.6%对 3 8.0 % ,P =0 .0 5 2 )。结论　 (1)XRCC1 3 99Arg/Arg可能具有潜在抵抗HCC发生的作用 ;(2 )XRCC1-3Objective To investigate the correlation between a single nucleotide polymorphism (SNP) of XRCC_1-399, a human NDA repair gene, and the development of primary hepatocytic carcinoma (HCC) in a case-control study.Methods The XRCC_1-399 SNP was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 72 HCC patients with pathological evidences and 137 matched control subjects recruited by matching on living area, sex and age.Results (1) The XRCC_1-399 SNP and age were not associated with the HCC development. Among individuals with XRCC_1-399 Arg/Arg, the HCC development had an inverse correlation with age (P=(0.028)); (2) HBV infection was an overwhelming risk for the development of HCC (P=(0.007)). Of individuals with -399Gln/Gln or Arg/Gln, those with HBV infection had a greater risk than those without it ((25.7%) v.s. (5.3%), P=(0.047)). (3) The rates of HBV infection in subjects with XRCC_1-399 Arg/Arg and with -399Gln/Gln or Arg/Gln were similar ((36.6%) v.s. (38.0%), P=(0.052)).Conclusion (1) The allele of XRCC_1-399 Arg/Arg might potentially resist against HCC development. (2) The alleles of -399Gln/Gln or Arg/Gln paralleled with HBV infection had high risk for HCC development.

关 键 词：XRCC1 HCC HBV感染 受试者 原发性肝细胞癌 相关研究 年龄 单核苷酸多态性 SNP 病例-对照研究 

分 类 号：R735.7[医药卫生—肿瘤] R730.1[医药卫生—临床医学]