乙型肝炎病毒相关性肝细胞癌患者肝硬化组织与癌组织蛋白质组的差异  

Proteome differences between HBV related hepatocellular carcinoma tissue and cirrhotic tissue

作　　者：龙云铸[1] 范学工[1] 李宁[1] 黄宇琨[1] 

机构地区：[1]长沙中南大学湘雅医院感染病科,湖南省株洲市一医院感染内科410008

出　　处：《肝脏》2004年第4期238-240,共3页Chinese Hepatology

基　　金：湖南省卫生厅科研重点资助课题 (No :Z0 2 0 5)

摘　　要：目的　分析乙型肝炎病毒 (HBV )相关性肝细胞癌患者肝硬化组织与癌组织蛋白质组之间的差异 ,鉴定其中的差异蛋白质点。方法　应用双向聚丙烯酰胺凝胶电泳 ,对 12例肝细胞癌患者的肝细胞癌组织与肝硬化组织的蛋白质进行差异分析 ,应用基质辅助激光解吸离子化飞行时间质谱仪对差异蛋白质点进行了鉴定。结果　经肝细胞癌组织与癌周组织的差异对比分析 ,共发现有表达水平存在显著差异的 3 5个蛋白质点 ,14个差异蛋白质点得到了初步鉴定 ,其中 5个蛋白质既往文献中曾报道其与肝细胞癌变相关。结论　HBV相关性肝细胞癌蛋白质组与肝硬化组织蛋白质组之间存在差异 ;本实验研究中初步鉴定的差异蛋白质 ,可能有助于HBV相关性肝细胞癌的癌变机制。Objective To analyze proteome difference of hepatic tumorous and cirrhotic tissues from HBV related hepatocellular carcinoma (HCC), and identify the differential protein spots.Methods Differential proteome profiles of 12 tumorous tissues, 12 cirrhotic tissues were analyzed using two-dimensional polyacrylamide gel electrophoresis (2-DE), differential protein spots were further identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).Results Comparing the tumorous tissue with surrounding non-tumorous tissue, a significant change in expression level was found in 35protein spots, among them 14 proteins were identifyied preliminarily. Eight proteins have not been reported to be associated with HBV related HCC in the literature previously.Conclusion The proteins revealed in this study may be of use for studies about mechanisms of hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HBV related HCC.

关 键 词：肝细胞癌 肝硬化 患者 乙型肝炎病毒 HBV 相关性 癌组织 蛋白质组 治疗 鉴定 

分 类 号：R735.7[医药卫生—肿瘤] R575.2[医药卫生—临床医学]