猕猴桃果实乙烯受体基因Ad-ETR1的克隆及其表达  

Cloning and Expression of Ethylene Receptor Gene Ad-ETR1 from Kiwifruit

作　　者：魏绍冲[1] 陈昆松[1] 张玉[2] 张望舒[1] 丁建国[1] 

机构地区：[1]浙江大学果实分子生理与生物技术实验室/农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029 [2]浙江省农业科学院,杭州310021

出　　处：《植物生理与分子生物学学报》2004年第6期681-686,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究发展规划项目(973项目)(No.G2000046806)国家自然科学基金项目(No.30170660)浙江省自然科学基金重点项目(No.ZD0004)高等学校博士学科点专项科研基金(20040335022)资助。~~

摘　　要：以"布鲁诺"美味猕猴桃似(Actinidia deliciosa cv.Bruno)果实为材料,根据其它植物乙烯受体氨基酸保守区序列,设计简并引物,通过RT-PCR扩增出1个657bp大小的cDNA片段(Ad-ETR1),该片段编码219个氨基酸,与其它植物乙烯受体及其基因的氨基酸及核苷酸同源性在72％-90％之间。Northern杂交结果表明,猕猴桃果实成熟衰老进程中Ad-ETR1 mRNA的积累趋于增加。这种积累的最大值出现在乙烯进入跃变之后:乙烯处理可以促使Ad-ETR1 mRNA最大值提前出现,乙酰水杨酸(ASA)处理则显著抑制Ad-ETR1表达。According to the conserved amino acid sequence from ethylene receptors in other plants, a pair of degenerate primers was designed and a 657-bp cDNA fragment encoding an ethylene receptor fruit was obtained by RT-PCR from ripening kiwifruit (Actinidia deliciosa cv. Bruno) (Fig.1). The cDNA fragment encoding 219 amino acids was named Ad-ETR1, and its sequences shared high similarity at both nucle-otide and polypeptide level with the sequences from the plants of Arabidopsis, tomato, persimmon, avocado, citrus and peach (Fig.2, Table 1). Northern blot analysis indicated that the levels of Ad-ETR1 mRNA increased during kiwifruit ripening and reached peak at 144 h after treatment, then dropped immediately. The expression of Ad-ETR1 could be induced by ethyl-ene treatment, while acetylsalicylic acid (ASA) treatment inhibited its expression (Fig.4). In consistence with the changes of Ad-ETR1 mRNA, ethylene or ASA treatment had marked effects on the kiwifruit ethylene production and fruit softening (Fig.3). The significance of these results was discussed.

关 键 词：猕猴桃 果实成熟 乙烯受体 ASA 乙烯 

分 类 号：S663.4[农业科学—果树学]