家蚕原代细胞有丝分裂观察  

Mitosis in Primary Cultures of Silkworm

作　　者：丁裕斌[1] 潘敏慧[1] 肖仕全[1] 陈敏[1] 洪锡钧[1] 鲁成[1] 

机构地区：[1]西南农业大学蚕学与生物技术学院农业部蚕桑学重点实验室,重庆400716

出　　处：《细胞生物学杂志》2004年第6期623-626,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金资助项目(No.30471312)国家高技术研究发展计划资助项目(863计划)(No.2004AA2Z1020)

摘　　要：在28℃,pH值6.4-6.8的条件下,用Grace培养基辅以20%的标准胎牛血清培养半消化法接种的家蚕晚期胚。原代培养细胞能够长期存活,观察到了家蚕原代培养过程中分化细胞的有丝分裂过程,发现了原代细胞分裂过程中赤道板的异常和染色体分离的异常现象;培养6个月和10个月的原代细胞分裂百分比分别为0.02±0.01和0.35±0.10,原代细胞异常的有丝分裂细胞百分比逐渐减少,分别为8.0±1.6和3.2±1.0。The explants used for primary culture are tissue and semi-digestive tissue, which originate from the late embryonic tissue of silkworm (Bombyx mori). The optimal conditions for primary culture are Grace's medium supplemented with moderate amount of antibiotics, 20% fetal bovine serum and incubated at temperature 28℃, pH 6.4-6.8. The whole process of mitosis and abnormal mitosis such as abnormal cell division plate and endonuclear mitosis were found when tissue had been cultured more than 6 months. With the time of culture prolonged, divide ratio increased from 0.02±0.01 to 0.35±0.10, while the percentage of abnormal mitosis decreased from 8.0±1.6 to 3.2±1.0.

关 键 词：家蚕 原代细胞 有丝分裂 体外培养 

分 类 号：Q813.11[生物学—生物工程]