用双极性膜电渗析法分离混合氨基酸  

Separation of mixed amino acids by bipolar membrane electrodialysis

作　　者：王辉[1] 余立新[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《清华大学学报（自然科学版）》2004年第12期1588-1591,共4页Journal of Tsinghua University(Science and Technology)

基　　金：国家"九七三"基础研究基金项目(2003CB615700)

摘　　要：为开发新的混合氨基酸分离技术,提出用双极性膜电渗析分离混合氨基酸的方法。在对混合氨基酸溶液中离子成分进行分析的基础上,预测了使用该法的可行性,并通过三室双极性膜电渗析法实验验证了理论计算的结果。对于等电点相差很大的谷氨酸和精氨酸以及等电点相差比较大的谷氨酸和甘氨酸以及精氨酸和甘氨酸两元混合物,可以实现很彻底的分离,得到纯度96%以上的产品,电流密度可以达到5～10mA.cm-2。对于等电点相差很小的丝氨酸和脯氨酸,则分离难度较大,电流密度在1mA.cm-2以下。该法不需使用缓冲溶液,可提高分离过程的效率。A membrane dialysis method was developed to separate amino acid mixtures. A bipolar membrane electrodialysis process was developed to separate amino acids based on calculated cation and anion concentrations in typical amino acid mixtures. Three-chambered electrodialysis experiments verified the prediction. For amino acids with very large isoelectric point differences, such as glutamic acid and arginine, or with moderate isoelectric point differences, such as glutamic acid and glycine or arginine and glycine, the separation process yields very pure amino acids with more than 96% purity. The current density ranges from 5～10 mA·cm^-2. For proline and serine which have a small isoelectric point difference, the separation is difficult to achieve and the current density is below 1 mA·cm^-2. Buffering solution is not needed in this process and the separation efficiency can be raised.

关 键 词：氨基酸 双极性膜 电渗析 分离 

分 类 号：O629.7[理学—有机化学]