实验性偏头痛动物模型c-fos、c-jun基因表达  

The Expression of c-fos and c-jun in the Experimental Migrainous Animal Model

作　　者：彭成[1] 任永欣[1] 姚干[1] 陈晓莉[1] 刘光谱[1] 王毅[1] 

机构地区：[1]成都中医药大学实验动物研究中心,成都610075

出　　处：《中国实验动物学报》2000年第2期112-119,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家"九五"攻关项目!(95 - 90 3- 0 10 1)

摘　　要：目的　复制实验性偏头痛动物模型并探讨其c -fos、c -jun基因表达。方法　采用CristinaTassorelli硝酸甘油法复制大鼠实验性偏头痛模型。免疫组化ABC法研究偏头痛大鼠脑组织即刻早期基因c -fos、c -jun的表达。结果　硝酸甘油型实验性偏头痛大鼠出现双耳发红、甩头、前肢频繁搔头 ,活动增加等外在表现 ,脑组织c -fos、c -jun基因表达阳性细胞数增加 ,基因表达阳性细胞的面积扩大、灰度降低或变化不大。结论　硝酸甘油型实验偏头痛大鼠模型复制方法简单、重复性较好、脑组织c -fos和c -jun基因异常表达明显 ,可以作为偏头痛治疗药物筛选、药效评价和发病机理研究的动物模型。Objective To produce an experimental migrainous animal model and probe the expression of c-fos and c-jun. Methods The nitroglycerin experimental migrainous animal model was reconstructed for the expression of c-fos and c-jun by means of immunohistochemical method Results The nitroglycerin experimental migrainous rats appeared reddish two ears, head shaking,scratching head with forepaws, activity increasing, both the number increasing and the area enlarging of the positive cells of the expression of c-fos and c-jun in brain tissues. Conclusion The model is a good one which can be used for evaluating drugs effect and studying pathogenesis.

关 键 词：偏头痛 实验性 C-FOS 表达 动物模型 硝酸甘油 脑组织 N基因 药效评价 阳性细胞 

分 类 号：Q786[生物学—分子生物学] R747.2[医药卫生—神经病学与精神病学]