DNA聚合酶-ⅠKlenow片段原位检测缺血性神经元的DNA早期断裂  

In situ detection of neuron DNA strand early breaks using the Klenow fragment of DNA polymerase -Ⅰ

作　　者：徐若华[1] 万琪[1] 王洪典[1] 李兵[2] 张巍[1] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032 [2]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华老年心脑血管病杂志》2000年第4期266-268,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的　探讨脑缺血 30min后DNA断裂损伤的发生时间。方法　用DNA聚合酶 ⅠKlenow大片段原位检测缺血性神经元的DNA早期断裂。结果　缺血再灌注 1h顶叶皮层、梨状皮层出现DNA断裂 ,再灌注 2h尾壳核出现DNA断裂 ,再灌注 2 4h ,各个区域的DNA断裂均达到顶峰。结论　DNA断裂损伤可能是缺血神经元改变的早期事件之一。Objective To investigate the time course of DNA strand breaks in rat brain following 30 min ischemia.Methods In situ detection of early neuronal DNA strand breaks by means of the Klenow Fragment of DNA polymerase Ⅰ mediated nick end labeling in the rat brain following 30 min ischemia and reperfusion.Results Klenow positive staining occurred in the parietal and piriform cortex within 1 h of reperfusion,in the caudate putamen within 2 h of reperfusion and most obviously within 24 h of reperfusion.Conclusion This results suggest that damage to nuclear DNA may be an early event after neuronal ischemia.

关 键 词：脑缺血 DNA损伤 Klenow 片段 

分 类 号：R743.3[医药卫生—神经病学与精神病学]