不同单位保存的艾氏腹水癌细胞(EAC)的某些生物学特性比较研究  

The Comparison Study on Biological Characteristics of EAC Cells from 3 Different Institutes

作　　者：徐增年[1] 王秀芳[1] 吕占军[1] 刘军须[1] 刘福英[1] 

机构地区：[1]河北医科大学实验动物学部,石家庄050017

出　　处：《中国实验动物学报》2001年第2期123-127,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家"九五"攻关课题! (96 A2 3 0 6 0 3 )

摘　　要：目的　比较研究不同单位保种的EAC瘤株生物学特性的差异。方法　选择研究了北京市肿瘤研究所、山东省医学科学院药物研究所及武汉大学保种中心保存EAC细胞的大小 ,细胞株体外培养时对血清的依赖性 ,接种小鼠皮下建立肿瘤动物模型肿块生长曲线 ,建立的动物模型对环磷酰胺CTX治疗的敏感性等指标。结果　发现三个单位保存的EAC细胞形态及大小基本一致 ;北京EAC体外培养容易生长 ,7 5%NCS即可生长良好 ,山东及武汉EAC体外培养生长力较弱 ;皮下接种BALB c小鼠后 ,北京EAC最易生长肿瘤 ,武汉EAC次之 ,山东EAC最差 ;建立的动物模型对CTX治疗的敏感性 ,依次为北京EAC ,武汉EAC ,山东EAC ,用CTX治疗的抑瘤率相应为 82 9% ,71 9% ,50 %。结果　可见三个不同单位保种的EAC瘤细胞生物学特性确已存在显著差异。Objective\ To compare biological characteristics of EAC cells from 3 different institutes.Methods\ Cell size was determined with the Cells Analysis System.The curve changes of neoplastic diameters were studied after subcutaneous injection of EAC in BALB/c.The neoplastic weights of the 12th day were measured and the sensitivity of EAC to CTX in BALB/c was compared.Results\ Both size and shape of cells were basicly of similarity.Ectogenesis of Beijing EAC was easier than the other two EAC. Induced tumors of Beijing EAC were the most by subcutaneous inoculation. The tumor-inhibitory rate to CTX treatment was 82.9%(Beijing EAC),71.9%(Wuhan EAC),50%(Shandong EAC) respectively. Conclusion\ The EAC cells from 3 different institutes appeared to be different markedly.\;

关 键 词：EAC 治疗 肿瘤 CTX 动物模型 体外培养 艾氏腹水癌 保种 生长 生物学特性 

分 类 号：Q959[生物学—动物学] R73-3[医药卫生—肿瘤]