HCMV大小鼠眼组织损伤模型的初步研究  

Study on Eye Disease of Animal Infected with HCMV AD_(169)Strain

作　　者：孟红[1] 孙广莲[1] 李焱[1] 刘菊华[1] 王玉梅[1] 

机构地区：[1]山东省医学科学院基础医学研究所微生物室,济南250062

出　　处：《中国实验动物学报》2001年第3期150-151,共2页Acta Laboratorium Animalis Scientia Sinica

基　　金：山东省自然科学基金资助课题 (Y96C160 48)

摘　　要：目的　探讨接种人巨细胞病毒 (Humancytomegalovirus ,HCMV)是否引起Wistar大鼠、昆明种小鼠眼组织损伤。方法　将HCMVAD1 6 9毒株经静脉接种Wistar大鼠和昆明种小鼠 ,观察动物眼部发病情况 ,原位杂交检测动物眼组织中的HCMVDNA片段。结果　接种病毒后部分动物缓慢出现眼部发病 ,局部分泌物增多、浑浊甚至失明 ;经原位杂交于视锥、视杆细胞和角膜内皮细胞中检出HCMVDNA片段。结论　HCMV可以感染动物眼组织 。Objective To investigate whether HCMV infection can cause ocular tissue lesions in rats and mice.Methods Twenty rats and ten mice were inoculated with HCMV AD 169 strain by vein and development of ocular disease was observed.HCMV DNA of tissues from inoculated animals were examined by in situ hybridization.Results Some animals expressed much secretion,turbidity in eyes even losing sight at different time post\|inoculation.HCMV DNA sections were identified in the corneal endothelium cell,rod cell and cone cell of the rats and mice inoculated. Conclusion HCMV AD 169 strain can infect rats and mice and cause ocular disease.\;

关 键 词：HCMV 小鼠 眼组织损伤 WISTAR大鼠 眼部 经静脉 原位杂交检测 动物 接种 毒株 

分 类 号：Q958[生物学—动物学] R779[医药卫生—眼科]