HBV DNA免疫的实验研究  

DNA based immunization against hepatitis B surface antigen in BALB/C mice

作　　者：刘东辉[1] 汪兴太[1] 汪德刚[1] 李长贵[1] 张华远[1] 李河民[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《临床输血与检验》1999年第3期1-3,51,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：从中国人血清中克隆出HBsAg adr亚型基因,采用pcDNA 3.1HisC真核细胞表达质粒进行基因重组。重组质粒pcDNA 3.1HisC-HBsAg分别用0.01、0.1、1、10和100μg腿部胫骨肌多点免疫BALB/C小鼠,4周时进行同样剂量加强免疫。初次免疫后4周和8周时分别检测HBsAg和HBsAb。结果表明,1μg以上剂量组加强免疫后,均可诱导小鼠产生特异性抗体。揭示HBV有发展DNA疫苗的可能性。HBsAg adr gene was cloned from serum of HBV carrier and reconstructed with pcDNA 3.1HisC which was designed for high-level expression and purification of recombinant proteins in Mammalian hosts. The BALB/C mice were injected with 0.01, 0.1, 1, 10 and 100μg recombinant pcDNA 3.1 HisC- HBsAg and enhanced immunization was given at 4 weeks interval. The results showed that protective level of specific humoral response was obtained by injecting more' than 1μg plasmid. It suggested the possibility of developing HBV DNA vaccine.

关 键 词：HBVDNA 加强免疫 实验研究 剂量 免疫后 真核细胞表达质粒 胫骨 克隆 重组质粒 基因重组 

分 类 号：R512.62[医药卫生—内科学] R446[医药卫生—临床医学]