早期及进展期胃癌DNA之检测——DNA含量与胃癌生长速度之研究  

Studies on the volume of DNA and the growth speed in early and advanced gastric carcinoma

作　　者：赵建业[1] 惠延平[1] 曹云新[2] 

机构地区：[1]第四军医大学二院病理科,西安710032 [2]第四军医大学中心实验室,西安710032

出　　处：《临床肿瘤学杂志》1997年第2期1-4,共4页Chinese Clinical Oncology

摘　　要：目的:研究DNA含量与胃癌细胞生长速度之关系。方法:Feulgen染色,用显微分光光度计,对胃癌各层细胞核内DNA分别进行定量检测。其中早期胃癌12例,未伴淋巴结转移9例,检测粘膜层癌细胞DNA,伴有淋巴结转移者3例,检测粘膜及淋巴结内癌细胞DNA;进展期癌11例,伴有淋巴结转移者4例,分别检测粘膜、肌层及淋巴结转移癌细胞DNA,不伴淋巴结转移7例,检测粘膜层,肌层癌细胞DNA。结果:早期胃癌细胞DNA含量明显低于进展期胃癌,统计学上有差异(P<0.01),DNA含量与有无转移无显著差异(P<0.05)。结论:DNA含量与胃癌生长速度密切相关。Purpose:To investigate the relationship between the volume of DNA and growth speed(GS) in gastric carcinoma. (GC)MethodS:Surgical resected GC specimen was used to study,each case has two sections,one for H. E. and another as Feulgen stain for DNA and counted under the German MPV Ⅱ type microspectrophotometer. There were twelve cases of early gastric car-cinoma (EGC) in which 9 cases no metastasis and counted the DNA of tumor cells in mucosa layer,in another 3 case counted the DNA in mucosa and lymph nodes. In all cases of advanced gastric carcinoma (AGC) 4 had metastasis in lymph nodes. We counted the tumor cell DNA in mucosa,muscularis and lymph node,in another 7 cases counted the DNA in mucosa and muscular layers. Results: The DNA in EGC lower than AGC,there was significant difference stastatically (P<0. 01). There was no statistically difference in the volume of tumor cell DNA and the metastasis of GC(P<0. 05). Conclusion:There was a close relationship be-tween the volume of tumor cell DNA and GS of GC.

关 键 词：DNA含量 胃癌细胞 癌生长 淋巴结转移癌 进展期胃癌 早期胃癌 显微 粘膜 定量检测 研究 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]