肺癌快速诊断渗滤试剂盒的研制及其临床应用  

Study on the filtration kit of rapid determination of lung cancer

作　　者：陈至善[1] 季桂芳[1] 张莉萍[1] 

机构地区：[1]解放军第455医院,上海200052

出　　处：《临床肿瘤学杂志》1997年第2期24-26,共3页Chinese Clinical Oncology

摘　　要：目的:为能简便快速检出肺癌。方法:研制以微孔滤膜为载体,以胶体金有色微粒为标记物的渗滤试剂盒。结果:检测肺癌血清141例,检出116例,漏诊25例;阳性率为82.3％,假阴性率为17.7％。检测良性肺疾患106例,检出肺癌阴性101例,阴性率为95.3％,其敏感性为82.3％,特异性为95.3％,准确性为87.9％。结论:其优点:不需加显色剂,检测快速简便;性能稳定,冷冻干燥后可在室温下长期保存;不需任何仪器设备,几分钟即可用肉眼判断结果。Purpose:To make the determination of lung cancer more simple and rapid. Methods: A new dot immunogold fil-tration assay(DIGFA) using micropore film as solid phase and colloidal gold as label was developed. Results: Tested with the es-tablished method,one hundred and sixteen of 141 cases with lung cancer were positive and one hundred and one of 106 patients with benign lung diseases were negative. The result shows that the sensitivity,specitivity and precision of the assay is 82. 3% ,95. 3% and 87. 9%,respectively. Conclusions:The study suggests that the filtration kit be rapid, simple and stable. The kit could be preserved permanently in the temperature of the room after freeze-drying. The performance could be finished without any special equipment and the result could be read with naked eye within a few minutes.

关 键 词：肺癌 临床应用 检出 试剂盒 快速诊断 肺疾患 阴性率 良性 假阴性 漏诊 

分 类 号：R734.2[医药卫生—肿瘤] R446[医药卫生—临床医学]