胃癌淋巴结转移与c-erbB-2蛋白、PCNA、DNA含量间关系的研究  

The relationship of lymph node metastasis in gastric cancer with the expression of c-erbB-2,PCNA and DNA contents

作　　者：陈至善[1] 徐建军[1] 熊显华[1] 

机构地区：[1]解放军第455医院,上海200052

出　　处：《临床肿瘤学杂志》1997年第3期14-16,共3页Chinese Clinical Oncology

摘　　要：目的:为探索胃癌淋巴结转移与c-erbB-2、PCNA(增殖细胞核抗原)、DNA含量间的关系。方法:应用免疫组化ABC法和流式细胞术DNA分析技术,检测胃癌标本64例,其中淋巴结转移组40例,淋巴结未转移组24例,结果:c-erbB-2蛋白表达两组阳性率分别为45％、20.8％(P<0.05);PCNA表达阳性率分别为51.1±18.7％、31.6±19.6％(P<0.01);DNA异倍体率分别为55％、20.8％(P<0.05)。结论:以上结果表明淋巴结转移组胃癌的c-erbB-2表达过度,产生大量生长因子或生长因子受体,促进细胞有丝分裂,处于增殖状态的细胞增多,致PCNA阳性率高,DNA异倍体率高,细胞间粘着力降低,致淋巴结易发生转移,生物学恶性度大,预后差。Puspose:To explore the relationship of lymph node metastasis in gastric cancer with c-erbB-2, PCNA (proliferat-ing cell naclear antigen) and DNA contents. Methods: 64 cases of gastric cancer were investigated with the methods of immunhis-tochemical ABC staining and flow cytometric DNA analysis. Among them, 40 cases had lymph node metastasis (┻), and 24 cases without lymph node metastasis (n-). Results: The results showed that the rates of positive c-erbB-2 and PCNA of n( + ) of n(┷) group were 45 and 51. 1?8. 7% respectively. which were significantly higher than those of n(-), in the n(-) group 20. 8 and 31. 6?9. 6% respectively (P<0. 05). The rates of DNA aneuploidy of nC + ) group was 55% on average,which was significantly higher than that of n(-) group, 20. 8%(P<0. 05). Conclusion:The results indicated that the gastric cancer tissue in n( + ) group had an over expression of c-erbB-2, and produced growth factor or growth factor receptor in large quantities,and stimulated cell mitosis, increased proliferating cells, raised the rates of PCNA positive and DNA aneuploidy and reduced the ad-herence to cells, and made the lymph nodes to be metastasized easily, lead to a high grade malignancy in biologic behaviour and a poor prognosis.

关 键 词：胃癌 PCNA 淋巴结转移 DNA含量 阳性率 C-ERBB-2蛋白 DNA异倍体 细胞 免疫组化ABC法 CERBB-2 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]