CD_(44)v6基因变异体在胃癌中的表达及其与淋巴结转移的关系  

Relationship Between the Expression of CD_(44).v6 and Lymph Node Metastasis in Gastric Carcinoma

作　　者：李泽民[1] 樊剑芳[1] 方建萍[1] 

机构地区：[1]上海铁道大学医学院附属甘泉医院病理科,200065

出　　处：《胃肠病学》1999年第4期210-212,共3页Chinese Journal of Gastroenterology

摘　　要：探讨胃癌中ＣＤ_（４４）ｖ６基因变异体的表达及其与淋巴结转移的关系。方法：采用免疫组织化学技术，对７５例手术切除胃癌标本中的ＣＤ_（４４）ｖ６基因变异表达产物进行检测。结果： ７５例胃癌标本中，５６例ＣＤ_（４４）ｖ６基因变异体呈阳性表达，占７４．７％；而５５例有淋巴结转移的标本中４６例呈阳性表达（８３．６％），２０例无淋巴结转移的标本中１０例呈阳性表达（５０％），差异有显著性（Ｐ＜０．００１）；正常胃粘膜不表达ＣＤ_（４４）ｖ６基因变异体。这一结果提示，ＣＤ_（４４）ｖ６基因变异体的表达与胃癌的淋巴结转移关系密切。结论：含ｖ６外显子的ＣＤ_（４４）变异表达产物可作为预测胃癌淋巴结转移潜能较可靠的分子标记物。Background/Aims: To investigate the role of the isoform (CD_(44)v6) of biomarker as possible prog- nostic antigen in gastric cancer and the relationship between CD_(44)v6 expression and metastatic behavior of the tumor. Methods: Immunohistochemistry technique was used to detect the distribution of CD_(44)v6 expression on formalin-fixed,paraffin-embedded tissue from resected specimens of gastric cancers of 75 patients. Results: 74.7% of cases (56/75) were positive for antiCD_(44)v6, but 25.3% of cases (19/75) were negative for antiCD_(44)v6.83.6% of cases (46/55) with lymph nodes metastasis and 50% of cases (10/20) without lymph node metastasis were positive for antiCD_(44)v6. Normal stomach mucosa was negative for CD_(44)v6. CD_(44)v6 expression revealed dramatic difference between the cases with and with- out lymph nodes metastasis (P<0.001), and the positive expression of CD_(44)v6 was closely associated with lymphatic metastasis. Conclusions: CD_(44)v6 appears to be a more reliable tumor marker for the early detection of metastatic potential in surgical biopsy specimens.

关 键 词：CD44V6基因 胃癌 淋巴结转移 阳性表达 变异体 标本 分子标记物 表达产物 外显子 显著性 

分 类 号：R735.2[医药卫生—肿瘤] R737[医药卫生—临床医学]