腈水合酶产生菌的分离、鉴定及部分酶学性质研究  

Screening and Identification of a Nitrile Hydratase Producing Bacterium Strain and Research of Enzymatic Properties

作　　者：邓林[1] 刘延岭[2] 王忠彦[1] 胡承[1] 孙勇[1] 段洪武[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省食品发酵设计院,成都610064

出　　处：《四川大学学报（自然科学版）》2005年第1期167-170,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：从长期被腈化物污染的土壤中筛选到一株腈水合酶产生菌YL 1.经过对分离菌株形态特征及生理生化指标的鉴定,该菌株初步鉴定为Rhodococcus.在含有丙烯腈的培养基中,细胞的生长与产物腈水合酶的合成相关.酶学性质研究表明:酶反应最适温度为30℃,最适pH为7 0.该酶在50℃以下,pH为6.0至9.0范围内较稳定.A strain YL-1,which had high degrading capacity for nitrile was isolated from soil polluted by nitrile.After identification with morphology and physiological characteristics of YL-1,we assumed that it might belong to the Rhodococcus. Acrylamide produced was paralleled with nitrile degradation during growth.Reaserch of enzymatic properties showed that the optimal temperature and pH were 30℃ and 70.This nitrile hydratase is stable under temperature 50℃ and between pH 60～90.

关 键 词：腈水合酶 红球菌 酶学性质 

分 类 号：Q93[生物学—微生物学]