针灸对克隆氏病大鼠结肠TNF-αIL-10mRNA的调节机制研究  

Study on the Modulating Mechanism of Colonic TNF-αand IL-10 mRNA Expression in CD rats with Acupuncture and Moxibustion

作　　者：施茵[1] 刘慧荣 华卫国[1] 秦秀娣[1] 吴焕淦[1] 钱睛兰[1] 

机构地区：[1]上海市针灸经络研究所,上海200030 [2]上海市气功研究所,上海200030

出　　处：《中医药学刊》2005年第2期272-275,共4页Study Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30 2 71 638)

摘　　要：目的 :探讨针灸对克隆氏病大鼠结肠TNF -α、IL -10mRNA的调节机制。方法 :在成功制备克隆氏病大鼠模型的基础上 ,随机分为模型组、隔药灸组、电针组三组 ,并与正常大鼠进行对照。隔药灸组、电针组选用“天枢”、“气海”穴分别进行隔药灸和电针治疗 ,以原位杂交法观察大鼠结肠组织TNF -α与IL -10mRNA的表达。结果 :克隆氏病模型大鼠结肠TNF -αmRNA与IL -10mRNA表达较正常组大鼠增高 ,隔药灸组、电针组均显著降低。结论 :针灸可能是通过抑制克隆氏病大鼠结肠组织促炎症细胞因子TNF -αmRNA的表达 ,调整IL -10mRNA与TNF -αmRNA之间的平衡 ,从而减轻或消除肠道炎症 ,以达治疗之目的。Objective: To observe the effect of acupuncture and moxibustion on the colonic expressions of TNF-αand IL-10mRNA in crohn's disease(CD)rats. Methods :The rats which were set up into CD's model were randomly divided into four groups including normal control group(NC),model control group(MC),herbs-partition moxibustion group(HPM)and electroapuncture group(EA).Tianshu(ST25,bilateral)and Qihai(RN6)were used in HPM and EA separately.The expressions of TNF-αand IL-10mRNA in colonic mucosa of CD rats were observed by situ hybridization. Results :The expressions of TNF-αand IL-10mRNA were markedly increased in CD rats compared with normal rats, and decreased significantly after herbs-partition moxibustion or electropuncture treatment. Conclusion :Acupuncture- moxibustion therapy can relieve or eliminate the intestinal inflammation maybe through effectively restraining the colonic expression of TNF-αmRNA, regulating the balance between IL-10mRNA and TNF-αmRNA in CD rats.

关 键 词：克隆氏病 TNF—α 隔药灸 大鼠 结肠 IL—10 RNA 结论 调节机制 目的 

分 类 号：R574[医药卫生—消化系统] R656.9[医药卫生—内科学]